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Silica-Coated, Waxberry-like Surface-Enhanced Raman Resonant Scattering Tag-Pair with Near-Infrared Raman Dye Encoding: Toward In Vivo Duplexing Detection

文献类型：期刊论文

作者Yin, Yingchao2,3; Mei, Rongchao3; Wang, Yunqing1,2,3; Zhao, Xizhen2,3; Yu, Qian3; Liu, Wanhui2; Chen, Lingxin1,3,4
刊名ANALYTICAL CHEMISTRY
出版日期2020-11-03
卷号92期号:21页码:14814-14821
ISSN号0003-2700
DOI10.1021/acs.analchem.0c03674
通讯作者Wang, Yunqing(yqwang@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要Surface-enhanced Raman resonant scattering (SERRS) tags encoded with near-infrared (NIR) Raman reporters showed great potential for in vivo detection owing to their ultrasensitivity. However, in vivo signal stability of such tags is a remaining problem due to the lack of suitable silica coating method because the weakly adsorbed NIR reporters tend to detach from traditional gold nanosubstrates in the ethanol-rich and high pH conditions, which are commonly used for silica coating. Herein, we propose a silica coating method for NIR SERRS tags by using waxberry-like gold nanoparticles (NPs) as substrates. The lipid bilayer of the NPs played a crucial role in the coating, which can encapsulate the NIR Raman reporter via hydrophobic interactions and prevent the interference from a harsh medium. Thus, the silica-coated tags well preserved ultrasensitivity of bare tags and simultaneously gained satisfactory signal stability in vivo. Moreover, the coating method is compatible for the encapsulation of a variety of thiol group-free NIR reporters (as exemplified by DTTC, Cy7, IR792, and DIR), relying on which a tag-pair with distinguishable peaks can be screened (labeling with DTTC and Cy7, respectively). In vivo duplexing detection revealed that the tag-pair-labeled liposome was cleared faster in the liver than polydopamine NPs within one mouse. The developed method paves an easy way for gaining high-quality SERRS tags and will promote their in vivo multiplex analysis and diagnostics applications.
资助项目National Natural Science Foundation of China[81573393] ; National Natural Science Foundation of China[21976209] ; National Natural Science Foundation of China[42076199] ; Science and Technology Development Plan of Shandong Province of China[2019GSF108047] ; Youth Innovation Promotion Association CAS[2017256] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201662] ; Taishan Scholar Project Special Funding[ts20190962] ; Research Initiation Fund of Binzhou Medical University[BY2019KYQD39] ; Technical Innovation Project of Instrument Function Development of the Chinese Academy of Sciences
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000589246100068
资助机构National Natural Science Foundation of China ; Science and Technology Development Plan of Shandong Province of China ; Youth Innovation Promotion Association CAS ; Instrument Developing Project of the Chinese Academy of Sciences ; Taishan Scholar Project Special Funding ; Research Initiation Fund of Binzhou Medical University ; Technical Innovation Project of Instrument Function Development of the Chinese Academy of Sciences
源URL[http://ir.yic.ac.cn/handle/133337/27516]  
专题海岸带生物学与生物资源利用重点实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
海岸带生物资源高效利用研究与发展中心
通讯作者Wang, Yunqing; Chen, Lingxin
作者单位1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
2.Yantai Univ, Collaborat Innovat Ctr Adv Drug Delivery Syst & B, Key Lab Mol Pharmacol & Drug Evaluat, Sch Pharm,Minist Educ, Yantai 264005, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China
4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Yin, Yingchao,Mei, Rongchao,Wang, Yunqing,et al. Silica-Coated, Waxberry-like Surface-Enhanced Raman Resonant Scattering Tag-Pair with Near-Infrared Raman Dye Encoding: Toward In Vivo Duplexing Detection[J]. ANALYTICAL CHEMISTRY,2020,92(21):14814-14821.
APA Yin, Yingchao.,Mei, Rongchao.,Wang, Yunqing.,Zhao, Xizhen.,Yu, Qian.,...&Chen, Lingxin.(2020).Silica-Coated, Waxberry-like Surface-Enhanced Raman Resonant Scattering Tag-Pair with Near-Infrared Raman Dye Encoding: Toward In Vivo Duplexing Detection.ANALYTICAL CHEMISTRY,92(21),14814-14821.
MLA Yin, Yingchao,et al."Silica-Coated, Waxberry-like Surface-Enhanced Raman Resonant Scattering Tag-Pair with Near-Infrared Raman Dye Encoding: Toward In Vivo Duplexing Detection".ANALYTICAL CHEMISTRY 92.21(2020):14814-14821.

入库方式： OAI收割

来源：烟台海岸带研究所

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