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SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing

文献类型：期刊论文

作者Bi, Liyan4; Wang, Xiao4; Cao, Xiaowei5; Liu, Luying4; Bai, Congcong4; Zheng, Qingyin4; Choo, Jaebum2; Chen, Lingxin1,3,4
刊名TALANTA
出版日期2020-12-01
卷号220页码:8
ISSN号0039-9140
关键词Au@AgNR SERS nanotag Antibiotic susceptibility testing Minimum inhibitory concentration Principal component analysis
DOI10.1016/j.talanta.2020.121397
通讯作者Bi, Liyan(liyan_bi@163.com) ; Choo, Jaebum(jbchoo@cau.ac.kr) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要There is a challenge to obtain an ultrasensitive and rapid approach to detect bacteria and identify resistance. As a powerful bioanalytical tool, surface-enhanced Raman scattering (SERS) in bacterial detection have attracted increasing attentions. Herein, we developed a SERS-active Au@Ag core-shell nanorod (Au@AgNR) tag platform for ultrasensitive bacteria detection and antibiotic-susceptibility testing (AST). The platform established that surface enhanced Raman scattered Rhodamine 6G (R6G) absorption at 1517 cm(-1) had a good linearity (RI = 3865 + 193logC; R-2 = 0.97) with logarithm of E. coli concentration over a range of 10(7)-10(2) CFU (colony forming unit)/mL with limit of detection as low 10(2) CFU/mL. When E. coli was exposed to ampicillin at minimum inhibitory concentration (MIC, 4 mu g/mL), Raman spectroscopy showed the obvious variation between ampicillin-susceptible E. coli (Amp(-)-E. coli) and the ampicillin-resistant E. coli (Amp(+)-E. coli). Combined with principal component analysis (PCA) statistical analysis, the Raman intensity variation mentioned above allows to obtain rapid antibiotic resistance testing (<3.5 h). In addition, E.coli spiked into blood from C57BL/6 mice can be identified clearly, indicating the potential for point-of-care diagnostics.
WOS关键词ENHANCED RAMAN-SCATTERING ; SENSITIVE DETECTION ; GOLD ; IDENTIFICATION ; SPECTROSCOPY ; DESIGN ; PROBE ; ARRAY
资助项目National Natural Science Foundation of China[21605005] ; National Natural Science Foundation of China[81530030] ; National Natural Science Foundation of China[81873697] ; National Natural Science Foundation of China[21976209] ; National Research Foundation of Korea[2019R1A2C3004375] ; National Research Foundation of Korea[2018M3A7B4071203] ; Research Initiation Fund of Binzhou Medical University[BY2019KYQD39] ; Taishan Scholar Project Special Funding[ts20190962]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000571477400006
资助机构National Natural Science Foundation of China ; National Research Foundation of Korea ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Funding
源URL[http://ir.yic.ac.cn/handle/133337/28396]  
专题烟台海岸带研究所_山东省海岸带环境工程技术研究中心
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者Bi, Liyan; Choo, Jaebum; Chen, Lingxin
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
2.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea
3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
4.Binzhou Med Univ, Transformat Otol & Neurosci Ctr, Yantai 264003, Peoples R China
5.Yangzhou Univ, Med Coll, Inst Translat Med, Yangzhou 225001, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714		 Bi, Liyan,Wang, Xiao,Cao, Xiaowei,et al. SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing[J]. TALANTA,2020,220:8.
APA Bi, Liyan.,Wang, Xiao.,Cao, Xiaowei.,Liu, Luying.,Bai, Congcong.,...&Chen, Lingxin.(2020).SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing.TALANTA,220,8.
MLA Bi, Liyan,et al."SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing".TALANTA 220(2020):8.

入库方式： OAI收割

来源：烟台海岸带研究所

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