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Gene expression concerning fatty acid and amino acid metabolism inChlorella vulgariscultured with antibiotics

文献类型：期刊论文

作者Wang, Wenjing; Sheng, Yanqing
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
出版日期2020-08-14
页码12
关键词Amino acid metabolism Axenic treatments Fatty acid metabolism Transcription analysis
ISSN号0175-7598
DOI10.1007/s00253-020-10822-6
通讯作者Sheng, Yanqing(yqsheng@yic.ac.cn)
英文摘要The green algaChlorella vulgarishas potential as a source of clean bioenergy with abundant metabolites and a high oil content, and antibiotics are often applied to remove bacteria from culture to obtain axenic algal strains. In this work, ceftazidime and gentamicin sulphate (GS) were added separately and in combination in the aseptic processing ofC. vulgaris, and gene expression and metabolites were evaluated. The results showed that ceftazidime and GS effectively inhibited the proliferation ofCyanobacteriaandBacteroidetes, respectively. Overall, the effects of antibiotics onC. vulgarisdiffered: GS increased the algal concentration, whereas ceftazidime alone and in combination with GS treatment decreased the specific algal growth rate. Based on comparative transcription analysis, 5917 and 5899 differentially expressed genes (DEGs) were respectively upregulated and downregulated by ceftazidime, 963 and 3921 DEGs by GS, and 4532 and 1675 DEGs by the ceftazidime and GS combination. Pathway enrichment analysis showed that the downregulated DEGs in the ceftazidime groups were enriched in the fatty acid biosynthesis pathway but that the upregulated DEGs in the GS group were enriched in the fatty acid degradation pathway. Some pathways related to amino acid metabolism were markably influenced by antibiotic treatment. The results further indicated that antibiotics affected the intracellular concentration of fatty acids and amino acids inC. vulgaris. This study provides a new viewpoint regarding the response ofC. vulgaristo antibiotics in the process of obtaining axenic algal strains.
WOS关键词CULTIVATION ; MICROALGAE ; TOXICITY ; BIOMASS ; ABILITY ; KEGG
资助项目National Natural Science Foundation of China[31600370] ; National Natural Science Foundation of China[41373100] ; Science and Technology Plan of Yantai City[2018ZHGY080] ; STS of the Chinese Academy of Sciences[KFJ-STS-QYZX-057]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000559428300001
资助机构National Natural Science Foundation of China ; Science and Technology Plan of Yantai City ; STS of the Chinese Academy of Sciences
源URL[http://ir.yic.ac.cn/handle/133337/28548]  
专题烟台海岸带研究所_山东省海岸带环境工程技术研究中心
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者Sheng, Yanqing
作者单位Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Wenjing,Sheng, Yanqing. Gene expression concerning fatty acid and amino acid metabolism inChlorella vulgariscultured with antibiotics[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2020:12.
APA Wang, Wenjing,&Sheng, Yanqing.(2020).Gene expression concerning fatty acid and amino acid metabolism inChlorella vulgariscultured with antibiotics.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,12.
MLA Wang, Wenjing,et al."Gene expression concerning fatty acid and amino acid metabolism inChlorella vulgariscultured with antibiotics".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020):12.

入库方式： OAI收割

来源：烟台海岸带研究所

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