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Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals

文献类型：期刊论文

作者Lv, Yingchun1,2; Zhang, Yang1,3; Song, Jiawei1,3; Xu, Gang1,2
刊名DESALINATION AND WATER TREATMENT
出版日期2020-02-01
卷号177页码:131-138
关键词Alkaline phosphatase Kinetic characteristics Heavy metal Swan Lake
ISSN号1944-3994
DOI10.5004/dwt.2020.24924
通讯作者Xu, Gang(gxu@yic.ac.cn)
英文摘要Phosphatase-catalyzed hydrolysis of organic phosphorus is an important pathway for providing bioavailable phosphorus to aquatic organisms. In this paper, a eutrophic water body was used to investigate how phosphorus and heavy metal ions affect alkaline phosphatase activity (APA) and its kinetic properties. The results show that phosphate (PO43-), hexametaphosphate ((PO3)(6)(6-)), pyrophosphate (P2O74-), and beta-glycerophosphate (C3H4O6P2-) inhibit APA and the inhibition effects increased with increasing concentration at the range of 0.01-1 mmol L-1. Addition of heavy metal ions, that is, Co2+, Pb2+, and Cr6+ at concentrations of 1 mmol L-1, increased the APA 3.1-fold, 2.3-fold, and 2.7-fold, respectively. The Cu2+, Zn2+, and Ni2+ exhibited an inhibitory effect on APA in water. For 1 mmol L-1 Cu2+, the APA deceased by 37.7% relative to the control. As the concentrations of Cu2+, PO43-, (PO3)(6)(6-), and P2O74- increased, the V-max of the alkaline phosphatase (AP) remained stable and K-m increased, indicating that these ions are competitive inhibitors of AP. In addition, V-max/K-m decreased with increasing concentrations of Cu2+, PO43-, (PO3)(6)(6-), P2O74-, and C3H4O6P2-, demonstrating the decreased catalytic efficiency of the AP. In summary, phosphorus and heavy metal significantly affect conversion of organic phosphorus into bioavailable inorganic phosphorous by changing the APA in eutrophic water bodies.
WOS关键词DISSOLVED ORGANIC PHOSPHORUS ; ENZYMES ; IMPACT
资助项目National Natural Science Foundation of China[41573120] ; National Natural Science Foundation of China[U1806215] ; Key Deployment Project of the Chinese Academy of Sciences[KFZD-SW-112] ; Science and Technology Service Network Initiative[KFJ-STS-ZDTP-023]
WOS研究方向Engineering ; Water Resources
语种英语
WOS记录号WOS:000544993600013
资助机构National Natural Science Foundation of China ; Key Deployment Project of the Chinese Academy of Sciences ; Science and Technology Service Network Initiative
源URL[http://ir.yic.ac.cn/handle/133337/28631]  
专题烟台海岸带研究所_近岸生态与环境实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者Xu, Gang
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China
2.Qufu Normal Univ, Coll Geog & Tourism, Rizhao 276826, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Lv, Yingchun,Zhang, Yang,Song, Jiawei,et al. Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals[J]. DESALINATION AND WATER TREATMENT,2020,177:131-138.
APA Lv, Yingchun,Zhang, Yang,Song, Jiawei,&Xu, Gang.(2020).Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals.DESALINATION AND WATER TREATMENT,177,131-138.
MLA Lv, Yingchun,et al."Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals".DESALINATION AND WATER TREATMENT 177(2020):131-138.

入库方式： OAI收割

来源：烟台海岸带研究所

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