中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 烟台海岸带研究所
  3. 中国科学院烟台海岸带研究所
  4. 中科院海岸带环境过程与生态修复重点实验室
Digital gene expression analysis in the gills of Ruditapes philippinarum after nitrite exposure

文献类型：期刊论文

作者Lv, Jiasen2; Cao, Tengfei2; Ji, Chenglong1,3,4; Cong, Ming3,4; Zhao, Jianmin3,4; Wu, Huifeng1,3,4
刊名ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
出版日期2019-11-30
卷号184页码:7
ISSN号0147-6513
关键词Digital gene expression Gills Ruditapes philippinarum Nitrite
DOI10.1016/j.ecoenv.2019.109661
通讯作者Cong, Ming(mcong@yic.ac.cn) ; Wu, Huifeng(hfwu@yic.ac.cn)
英文摘要Due to the overload of pollutants from highly intensive anthropic activities, nitrite accumulates in offshore seawater and has been a long-lasting pollutant to the healthy aquaculture of the mollusk. In the present study, Ruditapes philippinarum was used as the target bivalve to receive nitrite exposure at environmental concentration for 1 and 7 days. Differentially expressed genes (DEGs) were detected and analyzed by a digital gene expression (DGE) approach to describe the toxicity of nitrite on the bivalve at the gene level. In the N1 group, 185 DEGs were generated and enriched in six Gene Ontology (GO) terms, including oxidoreductase activity, heme binding, tetrapyrrole binding, iron ion binding, metal binding and cation binding. The DEGs in the N1 group were also enriched in two Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, e.g., arachidonic acid metabolism and ovarian steroidogenesis. In the N7 group, 81 DEGs were generated without any GO enrichment but were enriched in five KEGG pathways, including protein processing in the endoplasmic reticulum, protein export, prion diseases, thyroid hormone synthesis and arachidonic acid metabolism. This suggested that nitrite exposure might cause adverse effects to the clams in several aspects, including oxidative damage, depressed immunity, and disorders in cell proliferation, hormone metabolism and tissue regeneration. Evaluation of oxidative stress indicated that nitrite exposure actually induced redox state imbalance by enhancing the contents of thiobarbituric acid reactive substances (TBARSs) and glutathione (GSH), and the activity of glutathione peroxidase (GSH-PX) but not superoxide dismutase (SOD). These results will provide valuable gene references for further study on the toxicology mechanism of bivalves under environmental nitrite stress.
WOS关键词LIPID-PEROXIDATION ; IMMUNE-RESPONSE ; TRANSFERASE ; AMMONIA ; METABOLISM ; TOXICITY ; NITRATE ; KEGG
资助项目Natural Science Foundation of China[NSFC 41876135] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050102]
WOS研究方向Environmental Sciences & Ecology ; Toxicology
语种英语
WOS记录号WOS:000489085200020
资助机构Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences
源URL[http://ir.yic.ac.cn/handle/133337/29130]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
烟台海岸带研究所_近岸生态与环境实验室
海岸带生物资源高效利用研究与发展中心
海岸带生物学与生物资源利用重点实验室
通讯作者Cong, Ming; Wu, Huifeng
作者单位1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China
2.Yantai Univ, Biol Sch, Yantai 264005, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
4.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Lv, Jiasen,Cao, Tengfei,Ji, Chenglong,et al. Digital gene expression analysis in the gills of Ruditapes philippinarum after nitrite exposure[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2019,184:7.
APA Lv, Jiasen,Cao, Tengfei,Ji, Chenglong,Cong, Ming,Zhao, Jianmin,&Wu, Huifeng.(2019).Digital gene expression analysis in the gills of Ruditapes philippinarum after nitrite exposure.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,184,7.
MLA Lv, Jiasen,et al."Digital gene expression analysis in the gills of Ruditapes philippinarum after nitrite exposure".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 184(2019):7.

入库方式： OAI收割

来源：烟台海岸带研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。