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Magneto-controlled potentiometric assay for E. coli based on cleavage of peptide by outer-membrane protease T

文献类型：期刊论文

作者Zhang, Han1,2,3; Mou, Junsong1,2,3; Ding, Jiawang1,2,4,5; Qin, Wei1,2,4,5
刊名ELECTROCHIMICA ACTA
出版日期2021-07-10
卷号384页码:8
关键词Potentiometric Peptide Magnetic field Bacteria Outer-membrane protease T
ISSN号0013-4686
DOI10.1016/j.electacta.2021.138408
通讯作者Ding, Jiawang(jwding@yic.ac.cn)
英文摘要Rapid, sensitive and reliable Escherichia coli (E. coli) detection and identification are critically important to protect public health. Here, we describe a magneto-controlled potentiometric assay for specific detection of E. coli cells by making use of the E. coli outer-membrane protease T (OmpT). OmpT is an endopepti-dase that specifically cleaves peptide at dibasic sites. A rationally designed peptide serving as both OmpT substrate and potentiometric signal reporter was immobilized on magnetic beads. The rapid accumu-lation and extraction of peptide-functionalized magnetic beads on a polymeric membrane doped with an ion exchanger can be achieved using a magnetic force. The magnetic-field-assisted extraction of the peptide into the polymeric membrane ion-sensitive sensor, as confirmed by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, can lead to a rapid, stable and reproducible potential change. OmpT is capable of cleaving the positively charged peptide on the magnetic beads, thus resulting in charge density change. The change in charge density and subsequently the potential change can be readily detected and used for quantification of E. coli at levels down to 5.0 x 10(-3) CFU mL(-1). This work provides a versatile, rapid and reliable potentiometric method for E. coli detection. (C) 2021 Elsevier Ltd. All rights reserved.
WOS关键词ESCHERICHIA-COLI ; SUBSTRATE-SPECIFICITY ; WATER ; PROTAMINE ; PROTEINS ; BEADS
资助项目National Natural Science Foundation of China[41876108] ; National Natural Science Foundation of China[U2006208] ; Instrument Developing Project of the Chinese Academy of Sciences[Y728021021] ; Taishan Scholar Program of Shandong Province[tspd20181215] ; Taishan Scholar Program of Shandong Province[tsqn201909163] ; Key Research and Development Program of Yantai[2018ZHGY053]
WOS研究方向Electrochemistry
语种英语
WOS记录号WOS:000651772000003
资助机构National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Taishan Scholar Program of Shandong Province ; Key Research and Development Program of Yantai
源URL[http://ir.yic.ac.cn/handle/133337/29318]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_近岸生态与环境实验室
通讯作者Ding, Jiawang
作者单位1.YICCAS, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
5.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Han,Mou, Junsong,Ding, Jiawang,et al. Magneto-controlled potentiometric assay for E. coli based on cleavage of peptide by outer-membrane protease T[J]. ELECTROCHIMICA ACTA,2021,384:8.
APA Zhang, Han,Mou, Junsong,Ding, Jiawang,&Qin, Wei.(2021).Magneto-controlled potentiometric assay for E. coli based on cleavage of peptide by outer-membrane protease T.ELECTROCHIMICA ACTA,384,8.
MLA Zhang, Han,et al."Magneto-controlled potentiometric assay for E. coli based on cleavage of peptide by outer-membrane protease T".ELECTROCHIMICA ACTA 384(2021):8.

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来源：烟台海岸带研究所

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