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Determination of chitosan content with ratio coefficient method and HPLC

文献类型：期刊论文

作者Miao, Qin1,4; Cui, Yulin1,4; Zhang, Jingjing1,4; Mi, Yingqi1,3,4; Tan, Wenqiang1,4; Li, Qing1,4; Gu, Guodong2; Dong, Fang1,4; Guo, Zhanyong1,3,4
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2020-12-01
卷号164页码:384-388
ISSN号0141-8130
关键词Ratio coefficient Determination of chitosan content Hydrolysis rate HPLC Average deacetylation degree (DD)
DOI10.1016/j.ijbiomac.2020.07.013
通讯作者Guo, Zhanyong(zhanyongguo@hotmail.com)
英文摘要The determination of chitosan is a significant topic that has been growing in importance for a variety of fields. To quantify chitosan, various analytical methods can be used. When high performance liquid chromatographic (HPLC) is used for quantitative analysis of chitosan, the chitosan is mainly hydrolyzed into glucosamine salts, which are determined by HPLC. Average deacetylation degree (DD) has an effect on acid hydrolysis of chitosan. In this paper, chitosan with different average DDs was hydrolyzed to observe its effect on the determination of chitosan content, and that the study of combining acid hydrolysis rates of chitosan (alpha) with different average DDs and chitin (beta) standard to form ratio coefficients (gamma) to quantify chitosan was designed. Then, the table about. values within certain average DD ranges of chitosan was obtained. By using. and a, the actual content of chitosan could be obtained simply and accurately. (C) 2020 Elsevier B.V. All rights reserved.
WOS关键词ACID-HYDROLYSIS ; LIQUID-CHROMATOGRAPHY ; DEACETYLATION ; GLUCOSAMINE ; CHITIN ; ACETYLATION
资助项目National Key Research and Development Programof China[2019YFD0900705] ; Key Research and Development Program of Shandong Province[2018YYSP027] ; Natural Science Foundation of Shandong Province of China[ZR2019BD064] ; Youth Innovation Promotion Association CAS[2020219]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:000588093700035
资助机构National Key Research and Development Programof China ; Key Research and Development Program of Shandong Province ; Natural Science Foundation of Shandong Province of China ; Youth Innovation Promotion Association CAS
源URL[http://ir.yic.ac.cn/handle/133337/27522]  
专题海岸带生物学与生物资源利用重点实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
烟台海岸带研究所_近岸生态与环境实验室
海岸带生物资源高效利用研究与发展中心
通讯作者Guo, Zhanyong
作者单位1.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Alliance Pharma Inc, 17 Lee Blvd, Malvern, PA 19355 USA
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Miao, Qin,Cui, Yulin,Zhang, Jingjing,et al. Determination of chitosan content with ratio coefficient method and HPLC[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,164:384-388.
APA Miao, Qin.,Cui, Yulin.,Zhang, Jingjing.,Mi, Yingqi.,Tan, Wenqiang.,...&Guo, Zhanyong.(2020).Determination of chitosan content with ratio coefficient method and HPLC.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,164,384-388.
MLA Miao, Qin,et al."Determination of chitosan content with ratio coefficient method and HPLC".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 164(2020):384-388.

入库方式： OAI收割

来源：烟台海岸带研究所

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