Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571
文献类型：期刊论文
| 作者 | Liu, Xiaolin3,4; Liu, Yanan3,4; Johnson, Kevin Scot5; Dong, Xiaoyan1,3; Xie, Zhihong1,2,3
|
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2020-12-07 |
| 卷号 | 11页码:12 |
| 关键词 | chemotaxis CheZ cellular localization Azorhizobium caulinodans rhizobia |
| ISSN号 | 1664-302X |
| DOI | 10.3389/fmicb.2020.585140 |
| 通讯作者 | Xie, Zhihong(zhxie@yic.ac.cn) |
| 英文摘要 | Chemotaxis is essential for the competitiveness of motile bacteria in complex and harsh environments. The localization of chemotactic proteins in the cell is critical for coordinating a maximal response to chemotactic signals. One chemotaxis protein with a well-defined subcellular localization is the phosphatase CheZ. CheZ localizes to cell poles by binding with CheA in Escherichia coli and other enteric bacteria, or binding with a poorly understood protein called ChePep in epsilon-Proteobacteria. In alpha-Proteobacteria, CheZ lacks CheA-binding sites, and its cellular localization remains unknown. We therefore determined the localization of CheZ in the alpha-Proteobacteria Azorhizobium caulinodans ORS571. A. caulinodans CheZ, also termed as CheZ(AC,) was found to be located to cell poles independently of CheA, and we suspect that either the N-terminal helix or the four-helix bundle of CheZ(AC) is sufficient to locate to cell poles. We also found a novel motif, AXXFQ, which is adjacent to the phosphatase active motif DXXXQ, which effects the monopolar localization of CheZ(AC). This novel motif consisting of AXXFQ is conserved in CheZ and widely distributed among Proteobacteria. Finally, we found that the substitution of phosphatase active site affects the polar localization of CheZ(AC). In total, this work characterized the localization pattern of CheZ containing a novel motif, and we mapped the regions of CheZ(AC) that are critical for its polar localization. |
| WOS关键词 | CHEMOTAXIS PHOSPHATASE CHEZ ; ESCHERICHIA-COLI ; POLAR LOCALIZATION ; PHOSPHORYLATED CHEY ; SIGNALING PROTEINS ; COMPLEX ; CHEMORECEPTOR ; BACTERIA ; BINDING ; DIVERSITY |
| 资助项目 | NSFC-Shandong Joint Fund Key Projects[U1806206] ; National Natural Science Foundation of China[31870020] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000600397400001 |
| 资助机构 | NSFC-Shandong Joint Fund Key Projects ; National Natural Science Foundation of China |
| 源URL | [http://ir.yic.ac.cn/handle/133337/27558]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | Xie, Zhihong |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 2.Shandong Agr Univ, Coll Resources & Environm, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Tai An, Shandong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China 4.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China 5.Univ Calif Santa Cruz, Dept Microbiol & Environm Toxicol, Santa Cruz, CA 95064 USA |
| 推荐引用方式 GB/T 7714 | Liu, Xiaolin,Liu, Yanan,Johnson, Kevin Scot,et al. Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571[J]. FRONTIERS IN MICROBIOLOGY,2020,11:12. |
| APA | Liu, Xiaolin,Liu, Yanan,Johnson, Kevin Scot,Dong, Xiaoyan,&Xie, Zhihong.(2020).Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571.FRONTIERS IN MICROBIOLOGY,11,12. |
| MLA | Liu, Xiaolin,et al."Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ in Azorhizobium caulinodans ORS571".FRONTIERS IN MICROBIOLOGY 11(2020):12. |
入库方式： OAI收割
来源：烟台海岸带研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。