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Carbon nanotubes accelerate acetoclastic methanogenesis: From pure cultures to anaerobic soils

文献类型：期刊论文

作者Xiao, Leilei2,3,6; Zheng, Shiling2,6; Lichtfouse, Eric4; Luo, Min1; Tan, Yang2; Liu, Fanghua2,3,5,6
刊名SOIL BIOLOGY & BIOCHEMISTRY
出版日期2020-11-01
卷号150页码:9
关键词Methane DIET-Independent methanogenesis Conductive material Acetoclastic methanogenesis Pure culture Proteome
ISSN号0038-0717
DOI10.1016/j.soilbio.2020.107938
通讯作者Liu, Fanghua(fhliu@yic.ac.cn)
英文摘要Direct interspecies electron transfer (DIET) between electricigens and methanogens has been shown to favour CO2 reduction to produce biomethane. Furthermore, DIET is accelerated by conductive materials. However, whether conductive materials can promote other methanogenic pathways is unclear due to a lack of detailed experimental data and the poor mechanistic studies. Here, we hypothesized that conductive carbon nanotubes (CNTs) stimulate acetoclastic methanogenesis independently of electricigens in pure cultures of Methanosarcina spp. and anaerobic wetland soil. We found a significant increase in the methane production rate during the growth phase, e.g. from 0.169 mM to 0.241 mM after addition of CNTs on the 3rd day. CNTs did not increase the abundance of electromicroorganisms or the electron transfer rate in anaerobic soils, using via microbial diversity and electrochemical analysis. C-13-CH3COOH labelling, stable carbon isotope fractionation and the CH3F inhibitor of acetoclastic methanogenesis were used to distinguish methanogenic pathways. CNTs mainly accelerated acetoclastic methanogenesis rather than CO2 reduction in both pure cultures and anaerobic soils. Furthermore, the presence of CNTs slightly alleviate the inhibition of CH3F on acetoclastic methanogenesis during the pure culture of Methanosarcina barkeri and Methanosarcina mazei with the production of more than 0.3 mM methane. CNTs closely attached to the cell surface were observed by transmission electron microscopy. Proteome analysis revealed a stimulation of protein synthesis with about twice the improvement involved in -COOH oxidation and electron transfer. Overall, our findings demonstrate that conducting CNTs favor methane production and that the mechanism involved is acetoclastic methanogenesis via acetate dismutation, at least partly, rather than classical CO2 reduction.
WOS关键词INTERSPECIES ELECTRON-TRANSFER ; METHANE PRODUCTION ; ISOTOPE FRACTIONATION ; ORGANIC-MATTER ; PADDY SOIL ; GROWTH ; CO2 ; COMMUNITIES ; BACTERIAL ; ACETATE
资助项目National Natural Science Foundation of China[41703075] ; National Natural Science Foundation of China[91751112] ; National Natural Science Foundation of China[41573071] ; Natural Science Foundation of Shandong Province[JQ201608] ; Natural Science Foundation of Shandong Province[ZR2016DQ12] ; Young Taishan Scholars Program of Shandong Province[tsqn20161054]
WOS研究方向Agriculture
语种英语
WOS记录号WOS:000581509300005
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Young Taishan Scholars Program of Shandong Province
源URL[http://ir.yic.ac.cn/handle/133337/28452]  
专题海岸带生物学与生物资源利用重点实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者Liu, Fanghua
作者单位1.Fuzhou Univ, Sch Environm & Resource, Fuzhou 350116, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
4.Aix Marseille Univ, Coll France, CEREGE, INRAE,IRD,CNRS, Ave Louis Philibert, F-13100 Aix En Provence, France
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
6.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Xiao, Leilei,Zheng, Shiling,Lichtfouse, Eric,et al. Carbon nanotubes accelerate acetoclastic methanogenesis: From pure cultures to anaerobic soils[J]. SOIL BIOLOGY & BIOCHEMISTRY,2020,150:9.
APA Xiao, Leilei,Zheng, Shiling,Lichtfouse, Eric,Luo, Min,Tan, Yang,&Liu, Fanghua.(2020).Carbon nanotubes accelerate acetoclastic methanogenesis: From pure cultures to anaerobic soils.SOIL BIOLOGY & BIOCHEMISTRY,150,9.
MLA Xiao, Leilei,et al."Carbon nanotubes accelerate acetoclastic methanogenesis: From pure cultures to anaerobic soils".SOIL BIOLOGY & BIOCHEMISTRY 150(2020):9.

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来源：烟台海岸带研究所

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