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In Vivo Molecular Insights into Syntrophic Geobacter Aggregates

文献类型：期刊论文

作者Wei, Wenchao3,4,5; Plymale, Andrew4; Zhu, Zihua1; Ma, Xiang2; Liu, Fanghua3; Yu, Xiao-Ying4
刊名ANALYTICAL CHEMISTRY
出版日期2020-08-04
卷号92期号:15页码:10402-10411
ISSN号0003-2700
DOI10.1021/acs.analchem.0c00653
通讯作者Liu, Fanghua(fhliu@yic.ac.cn) ; Yu, Xiao-Ying(xiaoying.yu@pnnl.gov)
英文摘要Direct interspecies electron transfer (DIET) has been considered as a novel and highly efficient strategy in both natural anaerobic environments and artificial microbial fuel cells. A syntrophic model consisting of Geobacter metallireducens and Geobacter sulfurreducens was studied in this work. We conducted in vivo molecular mapping of the outer surface of the syntrophic community as the interface of nutrients and energy exchange. System for Analysis at the Liquid Vacuum Interface combined with time-of-flight secondary ion mass spectrometry was employed to capture the molecular distribution of syntrophic Geobacter communities in the living and hydrated state. Principal component analysis with selected peaks revealed that syntrophic Geobacter aggregates were well differentiated from other control samples, including syntrophic planktonic cells, pure cultured planktonic cells, and single population biofilms. Our in vivo imaging indicated that a unique molecular surface was formed. Specifically, aromatic amino acids, phosphatidylethanolamine components, and large water clusters were identified as key components that favored the DIET of syntrophic Geobacter aggregates. Moreover, the molecular changes in depths of the Geobacter aggregates were captured using dynamic depth profiling. Our findings shed new light on the interface components supporting electron transfer in syntrophic communities based on in vivo molecular imaging.
WOS关键词ION MASS-SPECTROMETRY ; C-TYPE CYTOCHROME ; INTERSPECIES ELECTRON-TRANSFER ; TOF-SIMS ANALYSIS ; PROTEIN FILMS ; FATTY-ACIDS ; SURFACE ; BIOFILM ; PILI ; XPS
资助项目Pacific Northwest National Laboratory (PNNL) Earth and Biological Sciences Directorate (EBSD) mission seed Laboratory Directed Research and Development (LDRD) ; Yantai Institute of the Coastal Zone Research Chinese Academy of Sciences from the National Natural Science Foundation of China[91751112] ; Yantai Institute of the Coastal Zone Research Chinese Academy of Sciences from the National Natural Science Foundation of China[41573071] ; PNNL Alternate Sponsored Fellowship (ASF) ; Graduate Fellowship from University of Chinese Academy of Sciences ; DOE[DE-AC05-76RL01830]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000558761500031
资助机构Pacific Northwest National Laboratory (PNNL) Earth and Biological Sciences Directorate (EBSD) mission seed Laboratory Directed Research and Development (LDRD) ; Yantai Institute of the Coastal Zone Research Chinese Academy of Sciences from the National Natural Science Foundation of China ; PNNL Alternate Sponsored Fellowship (ASF) ; Graduate Fellowship from University of Chinese Academy of Sciences ; DOE
源URL[http://ir.yic.ac.cn/handle/133337/28545]  
专题海岸带生物学与生物资源利用重点实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者Liu, Fanghua; Yu, Xiao-Ying
作者单位1.Pacific Northwest Natl Lab, Environm & Mol Sci Lab, Richland, WA 99352 USA
2.Grand View Univ, Dept Chem, Des Moines, IA 50316 USA
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Shandong, Peoples R China
4.Pacific Northwest Natl Lab, Energy & Environm Directorate, Richland, WA 99352 USA
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wei, Wenchao,Plymale, Andrew,Zhu, Zihua,et al. In Vivo Molecular Insights into Syntrophic Geobacter Aggregates[J]. ANALYTICAL CHEMISTRY,2020,92(15):10402-10411.
APA Wei, Wenchao,Plymale, Andrew,Zhu, Zihua,Ma, Xiang,Liu, Fanghua,&Yu, Xiao-Ying.(2020).In Vivo Molecular Insights into Syntrophic Geobacter Aggregates.ANALYTICAL CHEMISTRY,92(15),10402-10411.
MLA Wei, Wenchao,et al."In Vivo Molecular Insights into Syntrophic Geobacter Aggregates".ANALYTICAL CHEMISTRY 92.15(2020):10402-10411.

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来源：烟台海岸带研究所

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