Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode
文献类型：期刊论文
| 作者 | Meng, Ling2; Wu, Ping2; Chen, Guoxiang1; Cai, Chenxin2; Sun, Yongming3; Yuan, Zhenhong3 |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2009-02-15 |
| 卷号 | 24期号:6页码:1751-1756 |
| 关键词 | Biosensor Dehydrogenase Coenzyme Glutamate Carbon nanotubes |
| ISSN号 | 0956-5663 |
| 中文摘要 | a glutamate biosensor based on the electrocatalytic oxidation of reduced nicotinamide adenine dinucleotide (nadh), which was generated by the enzymatic reaction, was developed via employing a single-walled carbon nanotubes/thionine (th-swnts) nanocomposite as a mediator and an enzyme immobilization matrix. the biosensor, which was fabricated by immobilizing glutamate dehydrogenase (gidh) on the surface of th-swnts, exhibited a rapid response (ca. 5 s), a low detection limit (0.1 mu m), a wide and useful linear range (0.5-400 mu m), high sensitivity (137.3 +/- 15.7) mu a mm(-1) cm(-2), higher biological affinity, as well as good stability and repeatability. in addition, the common interfering species, such as ascorbic acid, uric acid, and 4-acetamidophenol, did not cause any interference due to the use of a low operating potential (190 mv vs. nhe). the biosensor can be used to quantify the concentration of glutamate in the physiological level. the th-swnts system represents a simple and effective approach to the integration of dehydrogenase and electrodes, which can provide analytical access to a large group of enzymes for wide range of bioelectrochemical applications including biosensors and biofuel cells. |
| 英文摘要 | a glutamate biosensor based on the electrocatalytic oxidation of reduced nicotinamide adenine dinucleotide (nadh), which was generated by the enzymatic reaction, was developed via employing a single-walled carbon nanotubes/thionine (th-swnts) nanocomposite as a mediator and an enzyme immobilization matrix. the biosensor, which was fabricated by immobilizing glutamate dehydrogenase (gidh) on the surface of th-swnts, exhibited a rapid response (ca. 5 s), a low detection limit (0.1 mu m), a wide and useful linear range (0.5-400 mu m), high sensitivity (137.3 +/- 15.7) mu a mm(-1) cm(-2), higher biological affinity, as well as good stability and repeatability. in addition, the common interfering species, such as ascorbic acid, uric acid, and 4-acetamidophenol, did not cause any interference due to the use of a low operating potential (190 mv vs. nhe). the biosensor can be used to quantify the concentration of glutamate in the physiological level. the th-swnts system represents a simple and effective approach to the integration of dehydrogenase and electrodes, which can provide analytical access to a large group of enzymes for wide range of bioelectrochemical applications including biosensors and biofuel cells. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biophysics ; biotechnology & applied microbiology ; chemistry, analytical ; electrochemistry ; nanoscience & nanotechnology |
| 研究领域[WOS] | biophysics ; biotechnology & applied microbiology ; chemistry ; electrochemistry ; science & technology - other topics |
| 关键词[WOS] | amperometric biosensor ; dehydrogenase ; enzyme ; oxidase ; glucose ; brain ; probes ; sensor ; ions |
| 收录类别 | SCI |
| 资助信息 | national natural science foundation of china (20673057, 20773067);the program for new century excellent talents in university (net-06-0508), and the "863" project (2007aa05z154) |
| 原文出处 | http://www.sciencedirect.com/science/journal/09565663 |
| 语种 | 英语 |
| WOS记录号 | WOS:000263778000035 |
| 公开日期 | 2010-09-23 |
| 源URL | [http://ir.giec.ac.cn/handle/344007/3448]  |
| 专题 | 中国科学院广州能源研究所 |
| 作者单位 | 1.Nanjing Normal Univ, Coll Life Sci, Nanjing 210097, Peoples R China 2.Nanjing Normal Univ, Coll Chem & Environm Sci, Jiangsu Key Lab Biofunct Mat, Nanjing 210097, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Energy Convers, Biomass Energy Ctr, Guangzhou 510640, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, Ling,Wu, Ping,Chen, Guoxiang,et al. Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode[J]. biosensors & bioelectronics,2009,24(6):1751-1756. |
| APA | Meng, Ling,Wu, Ping,Chen, Guoxiang,Cai, Chenxin,Sun, Yongming,&Yuan, Zhenhong.(2009).Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode.biosensors & bioelectronics,24(6),1751-1756. |
| MLA | Meng, Ling,et al."Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode".biosensors & bioelectronics 24.6(2009):1751-1756. |
入库方式： OAI收割
来源：广州能源研究所
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