Amelioration of Enterotoxigenic Escherichia coli-Induced Intestinal Barrier Disruption by Low-Molecular-Weight Chitosan in Weaned Pigs is Related to Suppressed Intestinal Inflammation and Apoptosis
文献类型：期刊论文
| 作者 | Wan, J; Zhang, J; Wu, GZ; Chen, DW; Yu, B; Huang, ZQ; Luo, YH; Zheng, P; Luo, JQ; Mao, XB |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2019 |
| 卷号 | 20期号:14页码:- |
| 关键词 | TIGHT JUNCTIONS LACTOBACILLUS-PLANTARUM DIETARY FIBER MAST-CELLS DEATH PERMEABILITY MODULATION RECEPTORS PATHWAYS BACTERIA |
| ISSN号 | 1422-0067 |
| DOI | 10.3390/ijms20143485 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Enterotoxigenic Escherichia coli (ETEC) infection destroys the intestinal barrier integrity, in turn, disrupting intestinal homoeostasis. Low-molecular-weight chitosan (LMWC) is a water-soluble chitosan derivative with versatile biological properties. Herein, we examined whether LMWC could relieve ETEC-induced intestinal barrier damage in weaned pigs. Twenty-four weaned pigs were allotted to three treatments: (1) non-infected control; (2) ETEC-infected control; and (3) ETEC infection + LMWC supplementation (100 mg/kg). On day 12, pigs in the infected groups were administered 100 mL of ETEC at 2.6 x 10(9) colony-forming units/mL to induce intestinal barrier injury. Three days later, serum samples were obtained from all pigs, which were then slaughtered to collect intestinal samples. We evidenced that LMWC not only increased (P < 0.05) the occludin protein abundance but also decreased (P < 0.05) the interleukin-6, tumour necrosis factor-alpha, and mast cell tryptase contents, and the apoptotic epithelial cell percentages, in the small intestine of ETEC-infected pigs. Furthermore, LMWC down-regulated (P < 0.05) the small intestinal expression levels of critical inflammatory- and apoptotic-related genes, such as Toll-like receptor 4 (TLR4) and tumour necrosis factor receptor 1 (TNFR1), as well as the intra-nuclear nuclear factor-kappa B (NF-kappa B) p65 protein abundance, in the ETEC-infected pigs. Our study indicated a protective effect of LMWC on ETEC-triggered intestinal barrier disruption in weaned pigs, which involves the repression of intestinal inflammatory responses via blocking the TLR4/NF-kappa B signalling pathway and the depression of epithelial cell death via TNFR1-dependent apoptosis. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31960]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, J,Zhang, J,Wu, GZ,et al. Amelioration of Enterotoxigenic Escherichia coli-Induced Intestinal Barrier Disruption by Low-Molecular-Weight Chitosan in Weaned Pigs is Related to Suppressed Intestinal Inflammation and Apoptosis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(14):-. |
| APA | Wan, J.,Zhang, J.,Wu, GZ.,Chen, DW.,Yu, B.,...&He, J.(2019).Amelioration of Enterotoxigenic Escherichia coli-Induced Intestinal Barrier Disruption by Low-Molecular-Weight Chitosan in Weaned Pigs is Related to Suppressed Intestinal Inflammation and Apoptosis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(14),-. |
| MLA | Wan, J,et al."Amelioration of Enterotoxigenic Escherichia coli-Induced Intestinal Barrier Disruption by Low-Molecular-Weight Chitosan in Weaned Pigs is Related to Suppressed Intestinal Inflammation and Apoptosis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.14(2019):-. |
入库方式： OAI收割
来源：上海应用物理研究所
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