Citrate-assisted efficient local delivery of naked oligonucleotide into live mouse brain cells
文献类型：期刊论文
| 作者 | Zhou, HB; Zhang, SH; Lv, F; Sun, WZ; Wang, LH; Fan, CH; Li, J; Hu, J |
| 刊名 | CELL PROLIFERATION
 |
| 出版日期 | 2019 |
| 卷号 | 52期号:4页码:- |
| 关键词 | GOLD NANOPARTICLES DNA PROLIFERATION DISEASE |
| ISSN号 | 0960-7722 |
| DOI | 10.1111/cpr.12622 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Objectives Synthetic oligonucleotides have shown promise in brain imaging. However, delivery of oligonucleotides into live brain cells remains challenging. In this study, we aim to develop a facile yet efficient strategy for local delivery of oligodeoxynucleotide (ODN) to neural cells in live adult mouse brain. Materials and methods A fluorescence-labelled ODN was diluted with sodium citrate buffer (100 mmol/L, pH = 3). One microlitre of the mixture was injected into a live adult mouse brain. Six hours later, we sacrificed the mouse and prepared brain slices for microscopic imaging. Results We find that the use of sodium citrate buffer in the one-shot local delivery can improve the diffusion and cell entry efficiency of the unmodified ODN for dozens of times. Only 1 pmol ODN leads to hundreds of positively transferred brain cells. We reason that this promotion is due to the local acidic condition created by the citrate buffer, which leads to the protonation of the ODN and some membrane proteins, thus reduces the Coulomb repulsion between the ODN and the cell membrane. Based on this strategy, we demonstrate fluorescent microscopic imaging of brain cells in different brain regions including striatum, cortex, hippocampus and midbrain. Conclusions The citrate buffer can be used as an adjuvant for facile and effective local injection delivery of ODNs, which may provide a new tool for brain imaging. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/31997]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China; 2.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai 201800, Peoples R China; 3.Univ Chinese Acad Sci, Beijing, Peoples R China; 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 5.ShanghaiTech Univ, IHuman Inst, Shanghai, Peoples R China; 6.Chinese Acad Sci, Inst Neurosci, Shanghai, Peoples R China; 7.Chinese Inst Brain Res, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, HB,Zhang, SH,Lv, F,et al. Citrate-assisted efficient local delivery of naked oligonucleotide into live mouse brain cells[J]. CELL PROLIFERATION,2019,52(4):-. |
| APA | Zhou, HB.,Zhang, SH.,Lv, F.,Sun, WZ.,Wang, LH.,...&Hu, J.(2019).Citrate-assisted efficient local delivery of naked oligonucleotide into live mouse brain cells.CELL PROLIFERATION,52(4),-. |
| MLA | Zhou, HB,et al."Citrate-assisted efficient local delivery of naked oligonucleotide into live mouse brain cells".CELL PROLIFERATION 52.4(2019):-. |
入库方式： OAI收割
来源：上海应用物理研究所
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