DNA Framework-Programmed Cell Capture via Topology-Engineered Receptor-Ligand Interactions
文献类型：期刊论文
| 作者 | Li, M; Ding, HM; Lin, MH; Yin, FF; Song, L; Mao, XH; Li, F; Ge, ZL; Wang, LH; Zuo, XL |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2019 |
| 卷号 | 141期号:47页码:18910-18915 |
| 关键词 | CIRCULATING TUMOR-CELLS ORIGAMI NANOSTRUCTURES CANCER-CELLS RELEASE RECOGNITION |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/jacs.9b11015 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Receptor-ligand interactions (RLIs) that play pivotal roles in living organisms are often depicted with the classic keys-and-locks model. Nevertheless, RLIs on the cell surface are generally highly complex and nonlinear, partially due to the noncontinuous and dynamic distribution of receptors on extracellular membranes. Here, we develop a tetrahedral DNA framework (TDF)-programmed approach to topologically engineer RLIs on the cell membrane, which enables active recruitment-binding of clustered receptors for high-affinity capture of circulating tumor cells (CTCs). The four vertices of a TDF afford orthogonal anchoring of ligands with spatial organization, based on which we synthesized n-simplexes harboring 1-3 aptamers targeting epithelial cell adhesion molecule (EpCAM) that are overexpressed on the membrane of tumor cells. The 2-simplex with three aptamers not only shows increased binding affinity (similar to 19-fold) but prevents endocytosis by cells. By using 2-simplex as the capture probe, we demonstrate the high-efficiency CTC capture, which is challenged in real clinical breast cancer patient samples. This TDF-programmed platform thus provides a powerful means for studying RLIs in physiological settings and for cancer diagnosis. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/32025]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200127, Peoples R China; 2.Shanghai Jiao Tong Univ, Inst Mol Med, State Key Lab Oncogenes & Related Genes, Renji Hosp,Sch Med, Shanghai 200127, Peoples R China; 3.Soochow Univ, Sch Phys Sci & Technol, Ctr Soft Condensed Matter Phys & Interdisciplinar, Suzhou 215006, Peoples R China; 4.China Univ Geosci, Fac Mat Sci & Chem, Engn Res Ctr Nanogeomat, Minist Educ, Wuhan 430074, Hubei, Peoples R China; 5.Chinese Acad Sci, Div Phys Biol, Shanghai 201800, Peoples R China; 6.Chinese Acad Sci, Shanghai Inst Appl Phys, Key Lab Interfacial Phys & Technol, Bioimaging Ctr, Shanghai 201800, Peoples R China; 7.Nanjing Univ, Natl Lab Solid State Microstruct, Nanjing 210093, Jiangsu, Peoples R China; 8.Nanjing Univ, Collaborat Innovat Ctr Adv Microstruct, Dept Phys, Nanjing 210093, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, M,Ding, HM,Lin, MH,et al. DNA Framework-Programmed Cell Capture via Topology-Engineered Receptor-Ligand Interactions[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2019,141(47):18910-18915. |
| APA | Li, M.,Ding, HM.,Lin, MH.,Yin, FF.,Song, L.,...&Fan, CH.(2019).DNA Framework-Programmed Cell Capture via Topology-Engineered Receptor-Ligand Interactions.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,141(47),18910-18915. |
| MLA | Li, M,et al."DNA Framework-Programmed Cell Capture via Topology-Engineered Receptor-Ligand Interactions".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 141.47(2019):18910-18915. |
入库方式： OAI收割
来源：上海应用物理研究所
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