Quantitative Measurement of Spatial Effects of DNA Origami on Molecular Binding Reactions Detected using Atomic Force Microscopy
文献类型：期刊论文
| 作者 | Zhang, P; Wang, F; Liu, WJ; Mao, XH; Hao, CC; Zhang, Y; Fan, CH; Hu, J; Wang, LH; Li, B |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2019 |
| 卷号 | 11期号:24页码:21973-21981 |
| 关键词 | KINETICS SHAPES ARRAYS |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.9b01691 |
| 文献子类 | 期刊论文 |
| 英文摘要 | DNA origami is a ubiquitous nanostructure that can be used as a universal scaffold for constructing molecular motors, nanosensors, nanodrugs, and optical devices. Understanding the inherent heterogeneity of DNA origami structures is crucial for optimizing the design of high-efficiency nanosized-devices. Here, we investigated the spatial effects of the DNA origami on binding reactions using atomic force microscopy. Protein complexes formed more efficiently at the vertex and rim than on the surface of the DNA origami; surprisingly, the maximum difference in biotin-streptavidin binding efficiency was over 80%, and the change in the binding rate was approximately 40-fold, suggesting the presence of distinct microenvironments at different locations of the DNA origami. Our findings are not only useful for the potential applications of the DNA origami, but also for clarifying differences in nanomaterials caused by nonuniform distribution or defects. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/32174]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 4.Shaanxi Normal Univ, Coll Phys & Informat Technol, Lab Biophys & Biomed, Xian 710062, Shaanxi, Peoples R China; 5.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China; 6.Shanghai Jiao Tong Univ, Inst Mol Med, Renji Hosp, Sch Med, Shanghai 200240, Peoples R China; 7.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China; 8.East China Normal Univ, Sch Chem & Mol Engn, Shanghai Key Lab Green Chem & Chem Proc, Shanghai 200241, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, P,Wang, F,Liu, WJ,et al. Quantitative Measurement of Spatial Effects of DNA Origami on Molecular Binding Reactions Detected using Atomic Force Microscopy[J]. ACS APPLIED MATERIALS & INTERFACES,2019,11(24):21973-21981. |
| APA | Zhang, P.,Wang, F.,Liu, WJ.,Mao, XH.,Hao, CC.,...&Li, B.(2019).Quantitative Measurement of Spatial Effects of DNA Origami on Molecular Binding Reactions Detected using Atomic Force Microscopy.ACS APPLIED MATERIALS & INTERFACES,11(24),21973-21981. |
| MLA | Zhang, P,et al."Quantitative Measurement of Spatial Effects of DNA Origami on Molecular Binding Reactions Detected using Atomic Force Microscopy".ACS APPLIED MATERIALS & INTERFACES 11.24(2019):21973-21981. |
入库方式： OAI收割
来源：上海应用物理研究所
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