Solidifying framework nucleic acids with silica
文献类型：期刊论文
| 作者 | Jing, XX; Zhang, F; Pan, MC; Dai, XP; Li, J; Wang, LH; Liu, XG; Yan, H; Fan, CH |
| 刊名 | NATURE PROTOCOLS
 |
| 出版日期 | 2019 |
| 卷号 | 14期号:8页码:2416-2436 |
| 关键词 | DNA ORIGAMI FOLDING DNA NANOSTRUCTURES NANOPARTICLES ASSEMBLIES DELIVERY GROWTH |
| ISSN号 | 1754-2189 |
| DOI | 10.1038/s41596-019-0184-0 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Soft matter can serve as a template to guide the growth of inorganic components with well-controlled structural features. However, the limited design space of conventional organic and biomolecular templates restricts the complexity and accuracy of templated growth. In past decades, the blossoming of structural DNA nanotechnology has provided us with a large reservoir of delicate-framework nucleic acids with design precision down to a single base. Here, we describe a DNA origami silicification (DOS) approach for generating complex silica composite nanomaterials. By utilizing modified silica sol-gel chemistry, pre-hydrolyzed silica precursor clusters can be uniformly coated onto the surface of DNA frameworks; thus, user-defined DNA-silica hybrid materials with similar to 3-nm precision can be achieved. More importantly, this method is applicable to various 1D, 2D and 3D DNA frameworks that range from 10 to > 1,000 nm. Compared to pure DNA scaffolds, a tenfold increase in the Young's modulus (E modulus) of these composites was observed, owing to their soft inner core and solid silica shell. We further demonstrate the use of solidified DNA frameworks to create 3D metal plasmonic devices. This protocol provides a platform for synthesizing inorganic materials with unprecedented complexity and tailored structural properties. The whole protocol takes similar to 10 d to complete. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/32209]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Inst Mol Med, Shanghai, Peoples R China; 2.Univ Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys,Div Phys Biol, Chinese Acad Sci,Shanghai Synchrotron Radiat Faci, Shanghai, Peoples R China; 3.Univ Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys,Bioimaging Ctr, Chinese Acad Sci,Shanghai Synchrotron Radiat Faci, Shanghai, Peoples R China; 4.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Sch Chem & Chem Engn, Shanghai, Peoples R China; 5.Biodesign Inst, Ctr Mol Design & Biomimet, Tempe, AZ USA; 6.Arizona State Univ, Sch Mol Sci, Tempe, AZ USA; 7.East China Normal Univ, Shanghai Key Lab Green Chem & Chem Proc, Sch Chem & Mol Engn, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jing, XX,Zhang, F,Pan, MC,et al. Solidifying framework nucleic acids with silica[J]. NATURE PROTOCOLS,2019,14(8):2416-2436. |
| APA | Jing, XX.,Zhang, F.,Pan, MC.,Dai, XP.,Li, J.,...&Fan, CH.(2019).Solidifying framework nucleic acids with silica.NATURE PROTOCOLS,14(8),2416-2436. |
| MLA | Jing, XX,et al."Solidifying framework nucleic acids with silica".NATURE PROTOCOLS 14.8(2019):2416-2436. |
入库方式： OAI收割
来源：上海应用物理研究所
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