Visualizing mRNA in live mammalian cells
文献类型：期刊论文
| 作者 | Wang, ZJ; Liu, WH; Fan, CH; Chen, N |
| 刊名 | METHODS
 |
| 出版日期 | 2019 |
| 卷号 | 161页码:16-23 |
| 关键词 | LIVING CELLS SPLICE VARIANTS IMAGING RNA IN-VITRO APTAMER FLUORESCENCE DYNAMICS TRANSLATION TRANSCRIPTS LOCALIZATION |
| ISSN号 | 1046-2023 |
| DOI | 10.1016/j.ymeth.2019.03.008 |
| 文献子类 | 期刊论文 |
| 英文摘要 | mRNAs play essential role in gene expression and regulation. Direct visualization of specific intracellular mRNAs can provide key information about mRNA distribution and dynamics that accelerating study of their functionality. This highlights the necessity of characterizing mRNA in mammalian cells with good spatial and temporal resolution. Live cell imaging and tracking of mRNA remains a challenge due to their relatively short half-life and lack of versatile imaging probes. This review provides an overview of current methods of live-cell mRNA imaging, including exogenous hybridization probes and genetically encoded strategies. It then focuses on the development and application of aptamer initiated fluorescence complementation (AiFC) method for real-time imaging of mammalian mRNAs and includes a detailed protocol for observing 13-actin mRNA in HeLa cells. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/32274]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China 2.Shanghai Normal Univ, Dept Chem, Shanghai 200234, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang, ZJ,Liu, WH,Fan, CH,et al. Visualizing mRNA in live mammalian cells[J]. METHODS,2019,161:16-23. |
| APA | Wang, ZJ,Liu, WH,Fan, CH,&Chen, N.(2019).Visualizing mRNA in live mammalian cells.METHODS,161,16-23. |
| MLA | Wang, ZJ,et al."Visualizing mRNA in live mammalian cells".METHODS 161(2019):16-23. |
入库方式： OAI收割
来源：上海应用物理研究所
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