Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics
文献类型：期刊论文
| 作者 | Song, Fengge1 ; Shen, Yuanyuan1; Wei, Yangdao1; Yang, Chunrong3; Ge, Xiaolin1; Wang, Aimin1; Li, Chaoyang1; Wan, Yi1,2; Li, Jinghong3
|
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2021-03-15 |
| 卷号 | 176页码:8 |
| ISSN号 | 0956-5663 |
| 关键词 | Bacterial diagnostic SARS-CoV-2 Botulinum toxin Fluorescence resonance energy transfer Colorimetric detection Biosensors |
| DOI | 10.1016/j.bios.2020.112953 |
| 通讯作者 | Wan, Yi(993602@hainanu.edu.cn) |
| 英文摘要 | The rapid identification of pathogenic microorganisms plays a crucial role in the timely diagnosis and treatment strategies during a global pandemic, especially in resource-limited area. Herein, we present a sensitive biosensor strategy depended on botulinum neurotoxin type A light chain (BoNT/A LC) activated complex assay (BACA). BoNT/A LC, the surrogate of BoNT/A which embodying the most potent biological poisons, could serve as an ultrasensitive signal reporter with high signal-to-noise ratio to avoid common strong background response, poor stability and low intensity of current biosensor methods. A nanoparticle hybridization system, involving specific binding probes that recognize pathogenic 16S rRNAs or SARS-CoV-2 gene site, was developed to measure doublestranded biotinylated target DNA containing a single-stranded overhang using Fluorescence Resonance Energy Transfer (FRET)-based assay and colorimetric method. The method is validated widely by six different bacteria strains and severe acute respiratory related coronavirus 2 (SARS-CoV-2) nucleic acid, demonstrating a single cell or 1 aM nucleic acid detecting sensitivity. This detection strategy offers a solution for general applications and has a great prospect to be a simple instrument-free colorimetric tool, especially when facing public health emergency. |
| 资助项目 | Key Research and the Development Program of Hainan, China[ZDYF(XGFY)2020002] ; National Key Research and Development Program of China, China[2018YFD0900704] ; National Natural Science Foundation of China, China[41866002] ; Tsinghua University Initiative Scientific Research Program, China[2020Z99CFZ019] ; Shandong Key Laboratory of Corrosion Science, China[KLCS201910] ; Research Foundation of Hainan University, China[KYQD(ZR)1711] |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| WOS记录号 | WOS:000612675000002 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/169733]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Wan, Yi |
| 作者单位 | 1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Key Lab Trop Biol Resources, Marine Coll,Minist Educ,Sch Life & Pharmaceut Sci, 56 Renmin Rd, Haikou 570228, Hainan, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Tsinghua Univ, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Dept Chem, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Fengge,Shen, Yuanyuan,Wei, Yangdao,et al. Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics[J]. BIOSENSORS & BIOELECTRONICS,2021,176:8. |
| APA | Song, Fengge.,Shen, Yuanyuan.,Wei, Yangdao.,Yang, Chunrong.,Ge, Xiaolin.,...&Li, Jinghong.(2021).Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics.BIOSENSORS & BIOELECTRONICS,176,8. |
| MLA | Song, Fengge,et al."Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics".BIOSENSORS & BIOELECTRONICS 176(2021):8. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。