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Susceptibility and immune responses of hybrid snakehead (Channa maculata female x Channa argus male) following infection with snakehead fish vesiculovirus

文献类型：期刊论文

作者Cao, Pan1,2; Sun, Wei3; Zhang, Yanbing3; Zhou, Zicheng3; Zhang, Xiaojun3; Liu, Xiaodan3
刊名AQUACULTURE
出版日期2021-02-25
卷号533页码:10
关键词Hybrid snakehead Snakehead fish vesiculovirus Susceptibility Histopathological analysis Immune responses
ISSN号0044-8486
DOI10.1016/j.aquaculture.2020.736113
通讯作者Cao, Pan(at19890928@126.com) ; Liu, Xiaodan(liuxiaodan@yzu.edu.cn)
英文摘要Snakehead fish vesiculovirus (SHVV) was initially isolated from diseased hybrid snakehead, and could cause great economic losses in snakehead culture due to its high morbidity and mortality. In this study, the susceptibility, histopathological analysis, and host immune response of hybrid snakehead infected with SHVV were investigated. SHVV was highly pathogenic to hybrid snakehead, and the infected fish exhibited typical signs of acute haemorrhages, and enlarged visceral organs. Histopathological analysis showed that the major tissues of diseased fish exhibited obvious inflammatory responses to SHVV infection. Additionally, humoral immune factors such as superoxide dismutase (SOD), catalase (CAT), and acid phosphatase (ACP) were assayed as serum indicators to evaluate the immune responses induced in infected hybrid snakehead. Quantitative real-time PCR (qRT-PCR) was also performed to evaluate the expression patterns of immune-related genes (HSP70, IL-8, MHCI alpha, MHCII alpha, SOD1, ZAP7 theta, PKCO, LCK, and HLA-A) in tissues of liver, spleen, head kidney and intestine. The results showed significantly enhanced immune activities in hybrid snakehead after SHVV infection. Altogether, these data provided insights into the pathogenic mechanism of SHVV and host immune response, facilitating the future prevention and treatment of hybrid snakehead infected with SHVV.
资助项目National Natural Science Foundation of China[32002420] ; Natural Science Foundation of Jiangsu Province[BK20180915] ; Natural Science Foundation of Jiangsu Province[BK20190916] ; Yangzhou City-Yangzhou University Cooperation Foundation[YZ2019140] ; Natural Science Fund for College and Universities in Jiangsu Province[18KJB240003] ; Jiangsu Agriculture Science and Technology Innovation Found[CX(18)2012] ; 'Blue Project' of Yangzhou University
WOS研究方向Fisheries ; Marine & Freshwater Biology
语种英语
WOS记录号WOS:000605010000007
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/169895]  
专题中国科学院海洋研究所
通讯作者Cao, Pan; Liu, Xiaodan
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Yangzhou Univ, Inst Surface Engn, Yangzhou 225127, Jiangsu, Peoples R China
3.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714		 Cao, Pan,Sun, Wei,Zhang, Yanbing,et al. Susceptibility and immune responses of hybrid snakehead (Channa maculata female x Channa argus male) following infection with snakehead fish vesiculovirus[J]. AQUACULTURE,2021,533:10.
APA Cao, Pan,Sun, Wei,Zhang, Yanbing,Zhou, Zicheng,Zhang, Xiaojun,&Liu, Xiaodan.(2021).Susceptibility and immune responses of hybrid snakehead (Channa maculata female x Channa argus male) following infection with snakehead fish vesiculovirus.AQUACULTURE,533,10.
MLA Cao, Pan,et al."Susceptibility and immune responses of hybrid snakehead (Channa maculata female x Channa argus male) following infection with snakehead fish vesiculovirus".AQUACULTURE 533(2021):10.

入库方式： OAI收割

来源：海洋研究所

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