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iTRAQ-based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress

文献类型：期刊论文

作者Liu, Lijie1,3,4; Lin, Lidong3; Ma, Zengling2; Wang, Guangce4; Wu, Mingjiang2
刊名AQUACULTURE RESEARCH
出版日期2020-09-20
页码11
关键词high temperature iTRAQ proteomics Sargassum fusiforme
ISSN号1355-557X
DOI10.1111/are.14880
通讯作者Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要Global warming increases seawater temperature, causing high temperature stress to marine organisms, including algae. This study aimed to explore the global proteomic response ofSargassum fusiformeunder high temperature stress.Sargassum fusiformeseedlings were cultured in natural seawater for 24 hr and subjected to different temperatures (22 degrees C, control group; 27 degrees C and 32 degrees C, high temperature stress group) for 1, 3, 5 and 7 days. Changes in their membrane lipid peroxidation after high temperature stress were investigated. Proteomic changes in the air bladders ofS. fusiformewere analysed using isobaric tags for relative and absolute quantification, along with liquid chromatography-tandem mass spectrometry. Data were analysed using bioinformatics methods. Results showed that high temperature stress destroyed the cell membrane of the air bladders. Further, 28 and 53 differentially expressed proteins (DEPs) were found in the 27 degrees C and 32 degrees C treatment groups respectively. These DEPs were mainly involved in glycolysis, single-organism catabolism, purine nucleoside diphosphate metabolism and carbohydrate catabolism. In addition, DEPs were significantly enriched in 10 pathways, such as glycolytic process, biosynthesis of antibiotics, ribosome, biosynthesis of secondary metabolites and biosynthesis of amino acids. Proteomics analyses indicated that proteins associated with synthesis, folding, degradation, photosynthesis and energy and carbohydrate metabolism are differentially expressed under high temperature stress and normal conditions.
资助项目Major Fisheries and Agriculture Science and Technology Projects in Dongtou District of Wenzhou City[N2018Y03A] ; Science and Technology Plan Projects in Dongtou District of Wenzhou City[N2016Y20B]
WOS研究方向Fisheries
语种英语
WOS记录号WOS:000571665900001
出版者WILEY
源URL[http://ir.qdio.ac.cn/handle/337002/168807]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Wang, Guangce
作者单位1.Qiqihar Univ, Coll Life Sci & Agr Forestry, Qiqihar, Heilongjiang, Peoples R China
2.Wenzhou Univ, Coll Life & Environm Sci, Chashan Univ Town, Wenzhou, Zhejiang, Peoples R China
3.Marine & Fishery Dev Res Ctr Dongtou Dist Wenzhou, Wenzhou, Zhejiang, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Lijie,Lin, Lidong,Ma, Zengling,et al. iTRAQ-based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress[J]. AQUACULTURE RESEARCH,2020:11.
APA Liu, Lijie,Lin, Lidong,Ma, Zengling,Wang, Guangce,&Wu, Mingjiang.(2020).iTRAQ-based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress.AQUACULTURE RESEARCH,11.
MLA Liu, Lijie,et al."iTRAQ-based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress".AQUACULTURE RESEARCH (2020):11.

入库方式： OAI收割

来源：海洋研究所

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