Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Lv, Xinjia2,3,5 ; Li, Shihao2,3,4; Yu, Yang2,3,4; Zhang, Xiaojun2,3,4; Li, Fuhua1,2,3,4
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2020-10-01 |
| 卷号 | 105页码:393-404 |
| 关键词 | Antimicrobial peptides Crustin RNA interference Gill microbiota Litopenaeus vannamei |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2020.07.014 |
| 通讯作者 | Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn) |
| 英文摘要 | Gills as the respiratory tissue of crustacean encounter various threats in the environment. The immune defense in gills is essential to the health of shrimp. In the present study, we identified a novel gill-abundant crustin, LvCrustin II-1, from the shrimp Litopenaeus vannamei. The full-length open reading frame of LvCrustin II-1 is 522 bp, which encodes 173 amino acid residues. The amino acid sequence of LvCrustin II-1 contained a signal peptide, a glycine rich region, a cysteine rich region and a whey acidic protein (WAP) domain. The expression level of LvCrustin II-1 was significantly up-regulated at different time points after Vibrio parahaemolyticus immersion. When LvCrustin II-1 was silenced by dsRNA interference, the count of bacteria colonies increased significantly in the hepatopancreas of shrimp after V. parahaemolyticus immersion, which indicated that the infection progress of pathogenic bacteria was accelerated after LvCrustin II-1 knockdown. Compared with the microbiota of seawater, the lower proportion of aquatic bacteria and higher proportion of symbiont in the gills microbiota of shrimp indicated the bacterial colonization was modulated by the host. Knockdown of LvCrustin II-1 changed the proportion of some potential pathogens and aquatic bacteria, which supported the idea that the new identified crustin in the gills played important roles in modulation of the microbiota community in shrimp. The present data provided new insights into the multiple functions of crustin in the immunity of shrimp. |
| 资助项目 | National Natural Science Foundation of China[31830100] ; National Natural Science Foundation of China[31772880] ; National Natural Science Foundation of China[41776158] ; China Agricultural Research System[CARS-48] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000571746900005 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/168810]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Shihao; Li, Fuhua |
| 作者单位 | 1.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Xinjia,Li, Shihao,Yu, Yang,et al. Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2020,105:393-404. |
| APA | Lv, Xinjia,Li, Shihao,Yu, Yang,Zhang, Xiaojun,&Li, Fuhua.(2020).Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,105,393-404. |
| MLA | Lv, Xinjia,et al."Characterization of a gill-abundant crustin with microbiota modulating function in Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 105(2020):393-404. |
入库方式： OAI收割
来源：海洋研究所
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