A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae)
文献类型：期刊论文
| 作者 | Shan, Tifeng; Pang, Shaojun3; Wang, Xuemei; Li, Jing ; Li, Qianxi; Su, Li; Li, Xiaodong
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| 刊名 | JOURNAL OF PHYCOLOGY
 |
| 出版日期 | 2020-10-27 |
| 页码 | 6 |
| 关键词 | breeding kelp macroalgae permanent population quantitative trait loci seaweed |
| ISSN号 | 0022-3646 |
| DOI | 10.1111/jpy.13066 |
| 通讯作者 | Pang, Shaojun(sjpang@qdio.ac.cn) |
| 英文摘要 | Studies of quantitative trait loci based on genetic linkage maps require the establishment of a mapping population. Permanent mapping populations are more ideal than temporary ones because they can be used repeatedly. However, there has been no reported permanent sporophyte population of economically important kelp species. Based on the characteristics of the kelp life cycle, we proposed a method to establish, and then constructed experimentally, an "immortalized F-2" (IF2) population of Undaria pinnatifida. Doubled-haploid "female" and "male" sporophytes were obtained through the parthenogenesis of a female gametophyte clone and the selfing of a "monoicous" gametophyte clone (originally male), respectively, and they were used as the parents. The F-1 hybrid line was generated by crossing the female and male gametophytes derived from the respective female and male parents. Full-sibling F-2 gametophyte clones, consisting of 260 females and 260 males, were established from an F-1 hybrid sporophyte. Thirty-five females and 35 males were randomly selected and paired to give rise to an IF2 population containing 35 crossing lines. A parentage analysis using 10 microsatellite markers confirmed the accuracy of the IF2 population and indicated the feasibility of this method. This proposed method may be adapted for use in other kelp species, and thus, it will be useful for genetic studies of kelp. |
| 资助项目 | National Natural Science Foundation of China[41676128] ; Biological Resources Program from Chinese Academy of Sciences[KFJ-BRP-017-27] ; China Agriculture Research System[CARS-50] ; Taishan Scholar Program of Shandong Province ; Foundation for Huiquan Scholar of Institute of Oceanology, Chinese Academy of Sciences |
| WOS研究方向 | Plant Sciences ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000583511200001 |
| 出版者 | WILEY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/168977]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Pang, Shaojun |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shan, Tifeng,Pang, Shaojun,Wang, Xuemei,et al. A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae)[J]. JOURNAL OF PHYCOLOGY,2020:6. |
| APA | Shan, Tifeng.,Pang, Shaojun.,Wang, Xuemei.,Li, Jing.,Li, Qianxi.,...&Li, Xiaodong.(2020).A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae).JOURNAL OF PHYCOLOGY,6. |
| MLA | Shan, Tifeng,et al."A method to establish an "immortalized F-2" sporophyte population in the economic brown alga Undaria pinnatifida (Laminariales: Alariaceae)".JOURNAL OF PHYCOLOGY (2020):6. |
入库方式： OAI收割
来源：海洋研究所
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