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Immunostimulatory effect of N-2-hydroxypropyltrimethyl ammonium chloride chitosan-sulfate chitosan complex nanoparticles on dendritic cells

文献类型：期刊论文

作者Xu, Chaojie1,2,3; Xing, Ronge2,3; Liu, Song2,3; Qin, Yukun2,3; Li, Kecheng2,3; Yu, Huahua2,3; Li, Pengcheng2,3
刊名CARBOHYDRATE POLYMERS
出版日期2021
卷号251页码:11
ISSN号0144-8617
关键词Chitosan quaternary ammonium salt Sulfated chitosan Nanoparticles DCS cells Immunostimulatory activities
DOI10.1016/j.carbpol.2020.117098
通讯作者Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要In this study, we synthesized negatively charged chitosan sulfate and positively charged hydroxypropyltrimethyl ammonium chloride chitosan (HACC), and then prepared chitosan derivatives with positive and negative ions as nanoparticles (NPs) by ovalbumin encapsulation using the polyelectrolyte method. NPs with different substitution sites and molecular weights (MW) were prepared by varying conditions. We then determined the zeta potential average, diameter, encapsulation effect, and their immunostimulatory effects on dendritic cells (DCs). The results showed that chitosan-derivative NPs ranged in size from 153.33 to 320.90 nm; all NPs were positive, with charges ranging from 17.10 to 39.30 mV and the encapsulation rates of 65 %-75 %. Three NPs greatly promoted the expression and secretion of interleukin-6 (IL-6), tumor necrosis factor (TNF-alpha), and interleukin-1 beta (IL-1 beta) in DC cells: C2,3,6 chitosan sulfate-HACC (C2,3,6-HACC; 200 kDa), C3,6 chitosan sulfate-HACC (C3,6-HACC; 200 kDa) and C6 chitosan sulfate-HACC (C6-HACC; 50 kDa). We also found that 200-kDa C2,3,6-HACC and 50-kDa C6-HACC NPs greatly increased secretion of the major histocompatibility complex-II (MHC-II), CD40, CD80, and CD86, indicating that these NPs promote effective antigen presentation, further increasing immunity effects. Finally, we applied laser confocal photography and determined that NPs entered the cell to promote the regulation of cellular immune activity; this discovery lays a foundation for further research on their mechanism of their action. Therefore, C2,3,6-HACC and C6-HACC NPs have the potential as immunological adjuvants.
资助项目National Key R&D Program of China[2018YFC0311305] ; Key Research and Development Program of Shandong Province[2019GHY112015] ; Key Research and Development Program of Shandong Province[2019YYSP028]
WOS研究方向Chemistry ; Polymer Science
语种英语
出版者ELSEVIER SCI LTD
WOS记录号WOS:000582801500092
源URL[http://ir.qdio.ac.cn/handle/337002/169066]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Xing, Ronge; Li, Pengcheng
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Chaojie,Xing, Ronge,Liu, Song,et al. Immunostimulatory effect of N-2-hydroxypropyltrimethyl ammonium chloride chitosan-sulfate chitosan complex nanoparticles on dendritic cells[J]. CARBOHYDRATE POLYMERS,2021,251:11.
APA Xu, Chaojie.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2021).Immunostimulatory effect of N-2-hydroxypropyltrimethyl ammonium chloride chitosan-sulfate chitosan complex nanoparticles on dendritic cells.CARBOHYDRATE POLYMERS,251,11.
MLA Xu, Chaojie,et al."Immunostimulatory effect of N-2-hydroxypropyltrimethyl ammonium chloride chitosan-sulfate chitosan complex nanoparticles on dendritic cells".CARBOHYDRATE POLYMERS 251(2021):11.

入库方式： OAI收割

来源：海洋研究所

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