Characterization and function analysis of a Kazal-type serine proteinase inhibitor in the red claw crayfish Cherax quadricarinatus
文献类型：期刊论文
| 作者 | Wang, Yan1,6; Wang, Baojie1; Liu, Mei1; Jiang, Keyong1; Wang, Mengqiang2,4,5 ; Wang, Lei1,3,7
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| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 出版日期 | 2021 |
| 卷号 | 114页码:10 |
| 关键词 | Cherax quadricarinatus Kazal-type serine proteinase inhibitor Innate immunity |
| ISSN号 | 0145-305X |
| DOI | 10.1016/j.dci.2020.103871 |
| 通讯作者 | Wang, Mengqiang(wangmengqiang@ouc.edu.cn) ; Wang, Lei(leiwang@qdio.ac.cn) |
| 英文摘要 | Kazal-type serine proteinase inhibitors (KPIs) function in physiological and immunological processes requiring pmteinase action. In the present study, the first Cherax quadricarinatus KPI gene (designated CqKPI) was identified and characterized. The open reading frame of CqKPI contains 405 nucleotides and encodes a protein of 134 amino acids. CqKPI has two Kazal domains comprising 44 amino acid residues with the conserved amino acid sequence C-X3-C-X-7-C-X-6-Y-X-3-C-X-6-C-X-12-C. Each Kazal domain has six conserved cysteine residues, which can form a structural conformation of three pairs of disulfide bonds stabilizing the Kazal domain. CqKPI exhibited high similarity with previously identified KPIs from crayfish hemocytes. The results of tissue distribution showed that CqKPI had the highest expression level in hemocytes, and this was in agreement with phylogenic relationships. Recombinant CqKPI (rCqKPI) was hetemlogously expressed in Escherichia coli and purified for further study. The proteinase inhibition assays suggested that rCqKPI could potently inhibit elastase and weakly inhibit trypsin, subtilisin A, and proteinase K, but not a-chymotrypsin. It can firmly bind to Bacillus hwajinpoensis, Staphylococcus aureus, and Vibrio parahaemolyticus, with weak binding to Candida albicans. In addition, CqKPI inhibited bacterial secretory pmteinase activity and inhibited the growth of B. hwajinpoensis and C. albicans. These data suggest that CqKPI might be involved in anti-bacterial immunity, acting as an inhibitor of the proteinase cascade in the resistance to invasion of pathogens. |
| 资助项目 | National Key R&D Program of China[2019YFD0900401] ; STS program supporting project of Chinese Academy of Sciences of China[2019T3035] |
| WOS研究方向 | Fisheries ; Immunology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000588061100055 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/169105]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Mengqiang; Wang, Lei |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Natl Lab Marine Sci & Technol, Ctr Marine Mol Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Peoples R China 4.Ocean Univ China, Lab Trop Marine Germplasm Resources & Breeding En, SANYA Oceanog Inst, Sanya 572024, Peoples R China 5.Ocean Univ China, MOE Key Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yan,Wang, Baojie,Liu, Mei,et al. Characterization and function analysis of a Kazal-type serine proteinase inhibitor in the red claw crayfish Cherax quadricarinatus[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,114:10. |
| APA | Wang, Yan,Wang, Baojie,Liu, Mei,Jiang, Keyong,Wang, Mengqiang,&Wang, Lei.(2021).Characterization and function analysis of a Kazal-type serine proteinase inhibitor in the red claw crayfish Cherax quadricarinatus.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,114,10. |
| MLA | Wang, Yan,et al."Characterization and function analysis of a Kazal-type serine proteinase inhibitor in the red claw crayfish Cherax quadricarinatus".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 114(2021):10. |
入库方式： OAI收割
来源：海洋研究所
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