Proteoglycan from Bacillus sp. BS11 Inhibits the Inflammatory Response by Suppressing the MAPK and NF-kappa B Pathways in Lipopolysaccharide-Induced RAW264.7 Macrophages
文献类型：期刊论文
| 作者 | Wang, Qingchi1,2,3,4 ; Liu, Weixiang1,2,3,4; Yue, Yang1,2,3,4; Sun, Chaomin1,2,3,4; Zhang, Quanbin1,2,3,4
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| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2020-12-01 |
| 卷号 | 18期号:12页码:16 |
| 关键词 | Bacillus sp proteoglycan anti-inflammation macrophages |
| DOI | 10.3390/md18120585 |
| 通讯作者 | Zhang, Quanbin(qbzhang@qdio.ac.cn) |
| 英文摘要 | Inflammation is involved in the pathogenesis of many debilitating diseases. Proteoglycan isolated from marine Bacillus sp. BS11 (EPS11) was shown to have anticancer activity, but its anti-inflammatory potential remains elusive. In the present study, the anti-inflammatory effects and mechanism of EPS11 were evaluated using a lipopolysaccharide (LPS)-induced RAW264.7 macrophage model. Biochemical characterization showed that the total sugar content and protein content of EPS11 were 49.5% and 30.2% respectively. EPS11 was composed of mannose, glucosamine, galactosamine, glucose, galactose, rhamnose, and glucuronic acid. Its molecular weight was determined to be 3.06 x 10(5) Da. The protein determination of EPS11 was also performed. EPS11 displayed a strong anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages in vitro, which significantly suppressed inflammatory cytokines and mediators (such as NO, TNF-alpha, IL-6 and IL-1 beta, and COX-2). Western blot analysis indicated that EPS11 could downregulate the expression of many key proteins in mitogen-activated protein kinases (MAPKs) and transcription factor nuclear factor-kappa B (NF-kappa B) signaling pathways. In particular, EPS11 almost completely inhibited the expression of NF-kappa B P65, which indicated that EPS11 acted primarily on the NF-kappa B pathways. These findings offer new insights into the molecular mechanism underlying the anti-inflammatory effect of EPS11. |
| 资助项目 | China Ocean Mineral Resources RD Association[DY135-B2-14] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000602108800001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/169420]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, Quanbin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Dept Earth Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Qingchi,Liu, Weixiang,Yue, Yang,et al. Proteoglycan from Bacillus sp. BS11 Inhibits the Inflammatory Response by Suppressing the MAPK and NF-kappa B Pathways in Lipopolysaccharide-Induced RAW264.7 Macrophages[J]. MARINE DRUGS,2020,18(12):16. |
| APA | Wang, Qingchi,Liu, Weixiang,Yue, Yang,Sun, Chaomin,&Zhang, Quanbin.(2020).Proteoglycan from Bacillus sp. BS11 Inhibits the Inflammatory Response by Suppressing the MAPK and NF-kappa B Pathways in Lipopolysaccharide-Induced RAW264.7 Macrophages.MARINE DRUGS,18(12),16. |
| MLA | Wang, Qingchi,et al."Proteoglycan from Bacillus sp. BS11 Inhibits the Inflammatory Response by Suppressing the MAPK and NF-kappa B Pathways in Lipopolysaccharide-Induced RAW264.7 Macrophages".MARINE DRUGS 18.12(2020):16. |
入库方式： OAI收割
来源：海洋研究所
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