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Preparation and Neutralization Efficacy of Novel Jellyfish Antivenoms against Cyanea nozakii Toxins

文献类型：期刊论文

作者Li, Rongfeng1,3; Yu, Huahua1,3; Li, Aoyu1; Yu, Chunlin1,2; Li, Pengcheng1,3
刊名TOXINS
出版日期2021-02-01
卷号13期号:2页码:16
关键词jellyfish Cyanea nozakii antivenom AntiCnTXs F(ab’ )(2)-AntiCnTXs Fab-AntiCnTXs
DOI10.3390/toxins13020165
通讯作者Li, Rongfeng(rongfengli@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要Jellyfish stings are a common issue globally, particularly in coastal areas in the summer. Victims can suffer pain, itching, swelling, shock, and even death. Usually, hot water, vinegar, or alumen is used to treat the normal symptoms of a jellyfish sting. However, a specific antivenom may be an effective treatment to deal with severe jellyfish stings. Cyanea nozakii often reach a diameter of 60 cm and are responsible for hundreds of thousands of stings per year in coastal Chinese waters. However, there has been no specific C. nozakii antivenom until now, and so the development of this antivenom is very important. Herein, we collected C. nozakii antisera from tentacle extract venom immunized rabbits and purified the immunoglobulin (IgG) fraction antivenom (AntiCnTXs). Subsequently, two complete procedures to produce a refined F(ab')(2) type of antivenom (F(ab')(2)-AntiCnTXs) and Fab type of antivenom (Fab-AntiCnTXs) by multiple optimizations and purification were established. The neutralization efficacy of these three types of antivenoms was compared and analyzed in vitro and in vivo, and the results showed that all types of antibodies displayed some neutralization effect on the lethality of C. nozakii venom toxins, with the neutralization efficacy as follows: F(ab')(2)-AntiCnTXs >= AntiCnTXs > Fab-AntiCnTXs. This study describes the preparation of novel C. nozakii jellyfish antivenom preparations towards the goal of developing a new, effective treatment for jellyfish stings.
资助项目National Natural Science Foundation of China[41876164] ; National Natural Science Foundation of China[41776163] ; Natural Science Foundation of Shandong Province[ZR2019QD012] ; National Key Research and Development Program of China[2017YFE0111100-04]
WOS研究方向Food Science & Technology ; Toxicology
语种英语
WOS记录号WOS:000623280000001
出版者MDPI
源URL[http://ir.qdio.ac.cn/handle/337002/169591]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Li, Rongfeng; Li, Pengcheng
作者单位1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
推荐引用方式
GB/T 7714		 Li, Rongfeng,Yu, Huahua,Li, Aoyu,et al. Preparation and Neutralization Efficacy of Novel Jellyfish Antivenoms against Cyanea nozakii Toxins[J]. TOXINS,2021,13(2):16.
APA Li, Rongfeng,Yu, Huahua,Li, Aoyu,Yu, Chunlin,&Li, Pengcheng.(2021).Preparation and Neutralization Efficacy of Novel Jellyfish Antivenoms against Cyanea nozakii Toxins.TOXINS,13(2),16.
MLA Li, Rongfeng,et al."Preparation and Neutralization Efficacy of Novel Jellyfish Antivenoms against Cyanea nozakii Toxins".TOXINS 13.2(2021):16.

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来源：海洋研究所

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