中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
  4. 实验海洋生物学重点实验室
Genome-wide identification of chitinase genes in Thalassiosira pseudonana and analysis of their expression under abiotic stresses

文献类型：期刊论文

作者Cheng, Haomiao1,2,4; Shao, Zhanru1,4; Lu, Chang1,2,4; Duan, Delin1,3,4
刊名BMC PLANT BIOLOGY
出版日期2021-02-10
卷号21期号:1页码:14
ISSN号1471-2229
关键词Abiotic stress Chitinase Enzymatic activity Gene expression Gene family Thalassiosira pseudonana
DOI10.1186/s12870-021-02849-2
通讯作者Shao, Zhanru(zrshao@qdio.ac.cn) ; Duan, Delin(dlduan@qdio.ac.cn)
英文摘要BackgroundThe nitrogen-containing polysaccharide chitin is the second most abundant biopolymer on earth and is found in the cell walls of diatoms, where it serves as a scaffold for biosilica deposition. Diatom chitin is an important source of carbon and nitrogen in the marine environment, but surprisingly little is known about basic chitinase metabolism in diatoms.ResultsHere, we identify and fully characterize 24 chitinase genes from the model centric diatom Thalassiosira pseudonana. We demonstrate that their expression is broadly upregulated under abiotic stresses, despite the fact that chitinase activity itself remains unchanged, and we discuss several explanations for this result. We also examine the potential transcriptional complexity of the intron-rich T. pseudonana chitinase genes and provide evidence for two separate tandem duplication events during their evolution.ConclusionsGiven the many applications of chitin and chitin derivatives in suture production, wound healing, drug delivery, and other processes, new insight into diatom chitin metabolism has both theoretical and practical value.
资助项目National Natural Science Foundation of China[41806175] ; National Key R&D Program of China[2018YFD0900106] ; Youth project of Marine Biology and Biotechnology Laboratory in Pilot National Laboratory for Marine Science and Technology Project[YQ2018NO06]
WOS研究方向Plant Sciences
语种英语
出版者BMC
WOS记录号WOS:000619672000001
源URL[http://ir.qdio.ac.cn/handle/337002/169688]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Shao, Zhanru; Duan, Delin
作者单位1.Pilot Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Bright Moon Seaweed Grp Co Ltd, State Key Lab Bioact Seaweed Subst, Qingdao 266400, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Cheng, Haomiao,Shao, Zhanru,Lu, Chang,et al. Genome-wide identification of chitinase genes in Thalassiosira pseudonana and analysis of their expression under abiotic stresses[J]. BMC PLANT BIOLOGY,2021,21(1):14.
APA Cheng, Haomiao,Shao, Zhanru,Lu, Chang,&Duan, Delin.(2021).Genome-wide identification of chitinase genes in Thalassiosira pseudonana and analysis of their expression under abiotic stresses.BMC PLANT BIOLOGY,21(1),14.
MLA Cheng, Haomiao,et al."Genome-wide identification of chitinase genes in Thalassiosira pseudonana and analysis of their expression under abiotic stresses".BMC PLANT BIOLOGY 21.1(2021):14.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。