A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus)
文献类型：期刊论文
| 作者 | Liu, Yifan1,3,4,5; Liu, Qinghua1,4; Xu, Shihong1,4,5; Wang, Yanfeng1,4,5; Feng, Chengcheng1,4,5 ; Zhao, Chunyan1,4,5; Song, Zongcheng2; Li, Jun1,4,5
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| 刊名 | FRONTIERS IN MARINE SCIENCE
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| 出版日期 | 2021-01-11 |
| 卷号 | 7页码:17 |
| 关键词 | fish reproduction spermatogenesis factory farmed fish steroids Scophthalmus maximus |
| DOI | 10.3389/fmars.2020.592880 |
| 通讯作者 | Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| 英文摘要 | Turbot (Scophthalmus maximus) is an important marine fish both in Europe and North China. Although there are plenty of studies on the reproduction of turbot, the complete cytological process of spermatogenesis remains unclear. In this study, we investigated the submicroscopic structure of total 23 types of male germ cells throughout the breeding season, with a relatively complete process of the primary spermatocytes. We found that the spermatid tail formed early at Spermatid II, and there were at least 16 spherical mitochondria in the spermatozoa. The hepatosomatic index (HSI) and gonadosomatic index (GSI) both peaked during the breeding season. Preliminary analysis showed that the vitality of mature sperm was negatively correlated with the proportion of sperm deformity. The serum 3,5,3 '-triiodothyronine (T3), 5-hydroxytryptamine (5-HT), testosterone (T), 17 alpha,20 beta-Dihydroxy-4-pregnen-3-one (17 alpha,20 beta-DHP), and 17 beta-estradiol (E2) all increased during the maturity period, with the change of T content most noticeable. Whereas in the testis, an overall high level of 11-ketotestosterone (11-KT) was more remarkable. The expression and localization of androgen receptor (AR) mRNA showed that the AR was highly expressed at the stages of II (15 - 70 g), with a slight rebound at the mature stages [IV(2200 g) to V(2500 g)], whose change was ahead to the changes of T and 11-KT. Fluorescence in situ hybridization (FISH) analysis showed that the AR mainly distributed in but not limited to Sertoli cells. This study represents the most complete overview of the reproductive cycle and spermatogenesis of turbot, which provides an important reference for the reproduction research and the guidance of flatfish breeding. |
| 资助项目 | National Natural Science Foundation of China[31702321] ; National Natural Science Foundation of China[31572602] ; National Natural Science Foundation of China[31802319] ; Zhejiang Provincial Natural Science Foundation of China[LY20C190008] ; Shandong Province Major Science and Technology Innovation Project[2018SDKJ0302-4] ; Shandong Province Key RD Projects[2019GHY112013] ; Natural Science Foundation of Shandong Province[ZR2018BC053] ; National Key Research and Development Program[2018YFD0901204] ; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[GML2019ZD0402] ; Major Agricultural Application Technology Innovation Project of Shandong Province[SD2019YY011] ; Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0502-2] ; China Agriculture Research System[CARS-47] ; Major Science and Technology for Scientific and Technological Innovation Projects (Shandong)[2019JZZY020710] ; STS Project[KFZD-SW-106] ; STS Project[ZSSD-019] ; STS Project[2017T3017] ; STS Project[2019T3022] |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000611002200001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/170093]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Qinghua; Li, Jun |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 2.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai, Peoples R China 3.Zhejiang Ocean Univ, Natl Engn Res Ctr Marine Aquaculture, Zhoushan, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China 5.Southern Marine Sci & Engn Guangdong Lab, Guangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yifan,Liu, Qinghua,Xu, Shihong,et al. A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus)[J]. FRONTIERS IN MARINE SCIENCE,2021,7:17. |
| APA | Liu, Yifan.,Liu, Qinghua.,Xu, Shihong.,Wang, Yanfeng.,Feng, Chengcheng.,...&Li, Jun.(2021).A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus).FRONTIERS IN MARINE SCIENCE,7,17. |
| MLA | Liu, Yifan,et al."A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus)".FRONTIERS IN MARINE SCIENCE 7(2021):17. |
入库方式： OAI收割
来源：海洋研究所
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