A putative insulin receptor involved in immune response of Chinese mitten crab Eriocheir sinensis
文献类型：期刊论文
| 作者 | Wang, L.3,4 ; Chen, H.3; Qiu, L.3; Wang, L.1,2,3; Song, L.1,2,3
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| 刊名 | ISJ-INVERTEBRATE SURVIVAL JOURNAL
 |
| 出版日期 | 2020 |
| 卷号 | 18页码:1-10 |
| 关键词 | Aeromonas hydrophila infection chinese mitten crab insulin receptor immune response |
| ISSN号 | 1824-307X |
| 通讯作者 | Song, L.(lshsong@dlou.edu.cn) |
| 英文摘要 | Insulin plays important roles in metabolic homeostasis during environmental challenges. The insulin receptor is a key molecule to receive and transduce insulin signals. In the present study, a novel insulin receptor was identified from the Chinese mitten crab Eriocheir sinensis (designated as EsIR). The coding region of EsIR gene was 3573 bp in length and encoded 1190 amino acids with all the functional domains of mammal insulin receptors, including furin-like domain, receptor L domain, transmembrane domain, and tyrosine kinase domain. Phylogenetic analysis showed that the EsIR shared the closest evolutionary relationship with the insulin receptor from Macrobrachium rosenbergii. Cell transfection experiments confirmed that EsIR proteins were localized on the cytomembrane. The mRNA transcripts of EsIR were widely distributed in various tissues with higher abundance in hepatopancreas and eyestalk of E. sinensis. After Aeromonas hydrophila stimulation, the expression level of EsIR mRNA decreased from 3 h to 6 h, and then increased at 12 h. The conserved structure and subcellular localization of EsIR together with its sensitivity to A. hydrophila stimulation implied that EsIR was probably involved in immune response of E. sinensis. The present study provided clues for the further investigation about the evolution and function of the insulin signaling pathway in invertebrates. |
| 资助项目 | National Key RD Program[2018YFD0900606] ; National Science Foundation of China[31530069] ; Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Talented Scholars in Dalian Ocean University |
| WOS研究方向 | Immunology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000614759900001 |
| 出版者 | INVERTEBRATE SURVIVAL JOURNAL |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/170105]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Song, L. |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China 2.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, L.,Chen, H.,Qiu, L.,et al. A putative insulin receptor involved in immune response of Chinese mitten crab Eriocheir sinensis[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2020,18:1-10. |
| APA | Wang, L.,Chen, H.,Qiu, L.,Wang, L.,&Song, L..(2020).A putative insulin receptor involved in immune response of Chinese mitten crab Eriocheir sinensis.ISJ-INVERTEBRATE SURVIVAL JOURNAL,18,1-10. |
| MLA | Wang, L.,et al."A putative insulin receptor involved in immune response of Chinese mitten crab Eriocheir sinensis".ISJ-INVERTEBRATE SURVIVAL JOURNAL 18(2020):1-10. |
入库方式： OAI收割
来源：海洋研究所
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