A Crustin from Hydrothermal Vent Shrimp: Antimicrobial Activity and Mechanism
文献类型：期刊论文
| 作者 | Wang, Yujian1,2,3,4 ; Zhang, Jian1,4,5; Sun, Yuanyuan1,2,4; Sun, Li1,2,3,4
|
| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2021-03-01 |
| 卷号 | 19期号:3页码:15 |
| 关键词 | crustin antimicrobial peptides shrimp deep-sea hydrothermal vent |
| DOI | 10.3390/md19030176 |
| 通讯作者 | Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | Crustin is a type of antimicrobial peptide and plays an important role in the innate immunity of arthropods. We report here the identification and characterization of a crustin (named Crus1) from the shrimp Rimicaris sp. inhabiting the deep-sea hydrothermal vent in Manus Basin (Papua New Guinea). Crus1 shares the highest identity (51.76%) with a Type I crustin of Penaeus vannamei and possesses a whey acidic protein (WAP) domain, which contains eight cysteine residues that form the conserved 'four-disulfide core' structure. Recombinant Crus1 (rCrus1) bound to peptidoglycan and lipoteichoic acid, and effectively killed Gram-positive bacteria in a manner that was dependent on pH, temperature, and disulfide linkage. rCrus1 induced membrane leakage and structure damage in the target bacteria, but had no effect on bacterial protoplasts. Serine substitution of each of the 8 Cys residues in the WAP domain did not affect the bacterial binding capacity but completely abolished the bactericidal activity of rCrus1. These results provide new insights into the characteristic and mechanism of the antimicrobial activity of deep sea crustins. |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403] ; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000633841100001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/170293]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Qingdao 266071, Peoples R China 5.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yujian,Zhang, Jian,Sun, Yuanyuan,et al. A Crustin from Hydrothermal Vent Shrimp: Antimicrobial Activity and Mechanism[J]. MARINE DRUGS,2021,19(3):15. |
| APA | Wang, Yujian,Zhang, Jian,Sun, Yuanyuan,&Sun, Li.(2021).A Crustin from Hydrothermal Vent Shrimp: Antimicrobial Activity and Mechanism.MARINE DRUGS,19(3),15. |
| MLA | Wang, Yujian,et al."A Crustin from Hydrothermal Vent Shrimp: Antimicrobial Activity and Mechanism".MARINE DRUGS 19.3(2021):15. |
入库方式： OAI收割
来源：海洋研究所
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