Derivation and Characterization of a New Embryonic Cell Line from the Olive Flounder Paralichthys olivaceus
文献类型：期刊论文
| 作者 | Nie, Miaomiao1,2,3,4; Wu, Zhihao2,4; You, Feng2,4 |
| 刊名 | TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
 |
| 出版日期 | 2021-04-01 |
| 卷号 | 21期号:4页码:159-167 |
| 关键词 | Paralichthys olivaceus Embryonic cell culture Kupffer's vesicle |
| ISSN号 | 1303-2712 |
| DOI | 10.4194/1303-2712-v21_4_01 |
| 通讯作者 | You, Feng(youfeng@qdio.ac.cn) |
| 英文摘要 | A new embryonic cell line that consisted predominantly of fibroblast cells has been established from embryos at Kupffer's vesicle (KV) stage of the olive flounder Paralichthys olivaceus, designated as the PoEKC. PoEKC cells have been subcultured for 61 passages over a period of 18 months. The cells were cultured in DMEM/F-12 medium supplemented with antibiotics, MS, and growth factors at temperature of 25 degrees C. The growth curve of the PoEKC cells comprised an exponential growth phase and a long plateau phase. Chromosome analysis revealed that the cells possessed the normal flounder diploid karyotype of 2n = 48t. The origin of the cell line was confirmed by testing the partial sequences of cytochrome oxidase c subunit I (COI) gene of mtDNA. The pluripotency markers, genes OCT4-1, SOX2, KLF4, and NANOG showed positive signals in the PoEKC cells, and the cells also presented high ALP activity. According to the above results, the PoEKC cells might be pluripotency, though the pluripotency needs further confirmation. The cells were also successfully transfected with GFP reporter gene suggesting that it could be utilized for gene function study in the flounder. |
| 资助项目 | National Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[31602143] ; National Key R&D Program of China[2018YFD0900202] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000629251700001 |
| 出版者 | CENTRAL FISHERIES RESEARCH INST |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/170299]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | You, Feng |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining, Peoples R China 4.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Key Lab Expt Marine Biol,Ctr Ocean Megasci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Nie, Miaomiao,Wu, Zhihao,You, Feng. Derivation and Characterization of a New Embryonic Cell Line from the Olive Flounder Paralichthys olivaceus[J]. TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES,2021,21(4):159-167. |
| APA | Nie, Miaomiao,Wu, Zhihao,&You, Feng.(2021).Derivation and Characterization of a New Embryonic Cell Line from the Olive Flounder Paralichthys olivaceus.TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES,21(4),159-167. |
| MLA | Nie, Miaomiao,et al."Derivation and Characterization of a New Embryonic Cell Line from the Olive Flounder Paralichthys olivaceus".TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES 21.4(2021):159-167. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。