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C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian

文献类型：期刊论文

作者Ma, Yuzhen1,3,4; Gao, Kun1,2,3,4; Yu, Huahua1,3,4; Liu, Weixiang1,3,4; Qin, Yukun1,3,4; Xing, Ronge1,3,4; Liu, Song1,3,4; Li, Pengcheng1,3,4
刊名CARBOHYDRATE POLYMERS
出版日期2021-06-01
卷号261页码:13
关键词Natural organic-based copper O-CSLn-Cu Phytophthora capsici Leonian Membrane damage and structural rearrangement Antifungal mechanism
ISSN号0144-8617
DOI10.1016/j.carbpol.2021.117821
通讯作者Yu, Huahua(yuhuahua@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要Damage to the cell membrane is an effective method to prevent drug resistance in plant fungal diseases. Here, we proposed a negative remodeling model of the cell membrane structure induced by the C-coordinated O-carboxymethyl chitosan Cu (II) complex (O-CSLn-Cu). FITC-labeled O-CSLn-Cu (FITC-O-CSLn-Cu) was first synthesized via a nucleophilic substitution reaction and confirmed by FT-IR. FITC-labeled O-CSLn-Cu could pass through the fungal cell membrane, as detected by confocal laser scanning microscopy (CLSM) coupled with fluorescein isothiocyanate (FITC)-fluorescence. O-CSLn-Cu treatment led to apparent morphological changes in the membranes of P. capsici Leonian and giant unilamellar vesicles (GUVs) by transmission electron microscopy (TEM). Then, we performed component analysis of the cell membrane from the P. capsici Leonian affected by OCSLn-Cu with a particular interest in membrane physicochemical properties. Many unsaturated fatty acids (UFAs) and key enzymes promoting UFA synthesis of the cell membrane were downregulated. Similarly, a large number of membrane proteins responsible for substance transport and biochemical reactions were downregulated. Furthermore, O-CSLn-Cu treatments increased plasma membrane permeability with significant leakage of intercellular electrolytes, soluble proteins and sugars, and lipid peroxidation with decreasing membrane fluidity. Finally, aquaporin 10 was proven to be a potential molecular target sensitive to antimicrobial agents according to composition analysis of membrane structure and immunohistochemistry.
资助项目National Key R&D Program of China[2018YFC0311300] ; National Key R&D Program of China[2019YFD0900705] ; National Natural Science Foundation of China[42076126]
WOS研究方向Chemistry ; Polymer Science
语种英语
WOS记录号WOS:000634108100006
出版者ELSEVIER SCI LTD
源URL[http://ir.qdio.ac.cn/handle/337002/170317]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Yu, Huahua; Li, Pengcheng
作者单位1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, CAS, Qingdao, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
推荐引用方式
GB/T 7714		 Ma, Yuzhen,Gao, Kun,Yu, Huahua,et al. C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian[J]. CARBOHYDRATE POLYMERS,2021,261:13.
APA Ma, Yuzhen.,Gao, Kun.,Yu, Huahua.,Liu, Weixiang.,Qin, Yukun.,...&Li, Pengcheng.(2021).C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian.CARBOHYDRATE POLYMERS,261,13.
MLA Ma, Yuzhen,et al."C-coordinated O-carboxymethyl chitosan Cu(II) complex exerts antifungal activity by disrupting the cell membrane integrity of Phytophthora capsici Leonian".CARBOHYDRATE POLYMERS 261(2021):13.

入库方式： OAI收割

来源：海洋研究所

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