Effects of busulfan on somatic cells after inhibiting germ cells in the gonads of the young olive flounder Paralichthys olivaceus
文献类型：期刊论文
| 作者 | Wang, Wenxiang1,2,3,4; Liang, Shaoshuai1,3,4; Zou, Yuxia1,3,4; Wang, Lijuan1,3,4 ; You, Feng1,3,4
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| 刊名 | ANIMAL REPRODUCTION SCIENCE
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| 出版日期 | 2021-05-01 |
| 卷号 | 228页码:10 |
| 关键词 | Olive flounder Paralichthys olivaceus Busulfan Expression Spermatogonium disruption Gonadal somatic cell |
| ISSN号 | 0378-4320 |
| DOI | 10.1016/j.anireprosci.2021.106746 |
| 通讯作者 | You, Feng(youfeng@qdio.ac.cn) |
| 英文摘要 | Busulfan is widely used in some species to inhibit germ cell proliferation. This study was conducted to evaluate effects of busulfan on germ and somatic cells in gonads of olive flounder, Paralichthys olivaceus, one of the most economically important mariculture fish species. After intraperitoneal injection with 80 (80B) or 120 (120B) mg/kg busulfan, both gonads were atrophied, and ovaries were discolored with adhesion to the visceral mass. Histological results indicated that germ cells in the gonads were detached, and there was a larger nucleus size and smaller cytoplasmic volume in spermatogonia. Numbers of oocytes and somatic cells in the ovary were both less (P < 0.05), while in the testis, numbers of spermatogonia and somatic cells were markedly lesser and greater, respectively (P < 0.05). In ovaries of the flounder treated with 80B and 120B, relative abundance of vasa and cyp19a1a mRNA transcripts was very small in the cytoplasm of oocytes, while the cyp19a1a transcript was still present in theca cells. In the testis of flounder treated with 80B and 120B, abundance of vasa was markedly less (P < 0.05) with there being very little vasa in spermatogonia and disruption of the spermatogonium structure. In the 80B treatment group, amh was in lesser abundance with there being very little amh in spermatogonia, however, with the 120B treatment there was a large amh abundance in spermatogonium with there being disruption of structure of these germ cells and Sertoli cells. Busulfan, therefore, might inhibit the development of spermatogonia in the flounder testis. |
| 资助项目 | National Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[31872558] ; National Natural Science Foundation of China[31702337] ; National Key R&D Program of China[2018YFD0900202] ; National Marine Genetic Resource Center |
| WOS研究方向 | Agriculture ; Reproductive Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000643550000007 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/170591]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | You, Feng |
| 作者单位 | 1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Wenxiang,Liang, Shaoshuai,Zou, Yuxia,et al. Effects of busulfan on somatic cells after inhibiting germ cells in the gonads of the young olive flounder Paralichthys olivaceus[J]. ANIMAL REPRODUCTION SCIENCE,2021,228:10. |
| APA | Wang, Wenxiang,Liang, Shaoshuai,Zou, Yuxia,Wang, Lijuan,&You, Feng.(2021).Effects of busulfan on somatic cells after inhibiting germ cells in the gonads of the young olive flounder Paralichthys olivaceus.ANIMAL REPRODUCTION SCIENCE,228,10. |
| MLA | Wang, Wenxiang,et al."Effects of busulfan on somatic cells after inhibiting germ cells in the gonads of the young olive flounder Paralichthys olivaceus".ANIMAL REPRODUCTION SCIENCE 228(2021):10. |
入库方式： OAI收割
来源：海洋研究所
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