Characterization of TCR+ and CD8(+) head kidney leucocytes in Japanese flounder (Paralichthys olivaceus) with antisera against TCR alpha and CD8 alpha
文献类型：期刊论文
| 作者 | Qiu, Reng3,4,5; Sun, Yuan Y.2; Guan, Cui C.3; Kan, Yun C.1,3,4,5; Yao, Lun G.3,4,5 |
| 刊名 | JOURNAL OF FISH BIOLOGY
 |
| 出版日期 | 2021-04-24 |
| 页码 | 9 |
| 关键词 | antiserum CD8α head kidney leucocytes Paralichthys olivaceus TCRα |
| ISSN号 | 0022-1112 |
| DOI | 10.1111/jfb.14722 |
| 通讯作者 | Kan, Yun C.(kanyunchao@163.com) ; Yao, Lun G.(lunguangyao@163.com) |
| 英文摘要 | T lymphocytes play an important role in cellular and adaptive immunity in vertebrates. The mechanisms of the fish immune system are little studied because of the lack of population-specific antibodies. This study examined the expression of two T lymphocyte markers, TCR alpha (PoTCR alpha) and CD8 alpha (PoCD8 alpha) in the Japanese flounder (Paralichthys olivaceus). The expression of PoTCR alpha and PoCD8 alpha was mainly detected in immune/mucosal tissues. Recombinant PoTCR alpha and PoCD8 alpha were expressed in pET32a and pET259, respectively. Then, rabbit anti-PoTCR alpha serum and rat anti-PoCD8 alpha serum were prepared. Using serum, the characteristics of TCR+ and CD8+ head kidney leucocytes (HKLs) were investigated. The results of laser scanning confocal microscopy (LSCM) demonstrated that TCR alpha and CD8 alpha were transmembrane proteins localized on the cell surface. The populations of CD8 alpha(-), CD8 alpha(+), TCR alpha(-), and TCR alpha(+) were sorted by flow cytometry (FCM) and analysed using qRT-PCR. The results demonstrated that all TCR alpha(+)/TCR alpha(-) or CD8 alpha(+)/CD8 alpha(-) HKLs expressed IFN-gamma. The CD4-1 and IgM transcripts were detected only in TCR alpha(-) and CD8 alpha(-) cells. Furthermore, HKL mitogenesis was induced with concanavalin A (ConA) stimulation. Taken together, the results from LSCM and FCM analyses showed that mammalian and P. olivaceus TCR+ and CD8(+) leucocytes share basic characteristics. |
| 资助项目 | Doctoral Research Project of Nanyang Normal University[ZX2014060] ; Henan Provincial Scientific and Technological Research Project[202102110109] ; National Natural Science Foundation of China[31870917] ; Program for Innovative Research Team of Science and Technology in University of Henan Province[20IRTSTHN024] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000643157000001 |
| 出版者 | WILEY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/170603]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Kan, Yun C.; Yao, Lun G. |
| 作者单位 | 1.Henan Univ, Sch Life Sci, Kaifeng 475000, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Nanyang Normal Univ, Henan Key Lab Ecol Secur Water Source Reg Mid Lin, Nanyang 473061, Peoples R China 4.Nanyang Normal Univ, Coll Life Sci & Agr Engn, Henan Prov Engn & Technol Ctr Hlth Prod Livestock, Nanyang 473061, Peoples R China 5.Nanyang Normal Univ, Coll Life Sci & Agr Engn, China UKNYNU RRes Joint Lab Insect Biol, Nanyang 473061, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, Reng,Sun, Yuan Y.,Guan, Cui C.,et al. Characterization of TCR+ and CD8(+) head kidney leucocytes in Japanese flounder (Paralichthys olivaceus) with antisera against TCR alpha and CD8 alpha[J]. JOURNAL OF FISH BIOLOGY,2021:9. |
| APA | Qiu, Reng,Sun, Yuan Y.,Guan, Cui C.,Kan, Yun C.,&Yao, Lun G..(2021).Characterization of TCR+ and CD8(+) head kidney leucocytes in Japanese flounder (Paralichthys olivaceus) with antisera against TCR alpha and CD8 alpha.JOURNAL OF FISH BIOLOGY,9. |
| MLA | Qiu, Reng,et al."Characterization of TCR+ and CD8(+) head kidney leucocytes in Japanese flounder (Paralichthys olivaceus) with antisera against TCR alpha and CD8 alpha".JOURNAL OF FISH BIOLOGY (2021):9. |
入库方式： OAI收割
来源：海洋研究所
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