Transcriptional Responses of the Heat Shock Protein 20 (Hsp20) and 40 (Hsp40) Genes to Temperature Stress and Alteration of Life Cycle Stages in the Harmful Alga Scrippsiella trochoidea (Dinophyceae)
文献类型：期刊论文
| 作者 | Deng, Yunyan1,2,3; Hu, Zhangxi1,2,3 ; Shang, Lixia1,2,3; Chai, Zhaoyang1,2,3; Tang, Ying Zhong1,2,3
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| 刊名 | BIOLOGY-BASEL
 |
| 出版日期 | 2020-11-01 |
| 卷号 | 9期号:11页码:17 |
| 关键词 | Scrippsiella trochoidea resting cyst dinoflagellate harmful algal blooms heat shock protein 20 (Hsp20) heat shock protein 40 (Hsp40) temperature stress |
| DOI | 10.3390/biology9110408 |
| 通讯作者 | Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn) |
| 英文摘要 | The small heat shock protein (sHsp) and Hsp40 are Hsp members that have not been intensively investigated but are functionally important in most organisms. In this study, the potential roles of a Hsp20 (StHsp20) and a Hsp40 (StHsp40) in dinoflagellates during adaptation to temperature fluctuation and alteration of different life stages were explored using the representative harmful algal blooms (HABs)-causative dinoflagellate species, Scrippsiella trochoidea. We isolated the full-length cDNAs of the two genes via rapid amplification of cDNA ends (RACE) and tracked their differential transcriptions via real-time qPCR. The results revealed StHsp20 and StHsp40 exhibited mRNA accumulation patterns that were highly similar in response to heat stress but completely different toward cold stress, which implies that the mechanisms underlying thermal and cold acclimation in dinoflagellates are regulated by different sets of genes. The StHsp20 was probably related to the heat tolerance of the species, and StHsp40 was closely involved in the adaptation to both higher and lower temperature fluctuations. Furthermore, significantly higher mRNA abundance of StHsp40 was detected in newly formed resting cysts, which might be a response to intrinsic stress stemmed from encystment. This finding also implied StHsp40 might be engaged in resting cyst formation of S. trochoidea. Our findings enriched the knowledge about possible cross-talk of different Hsp members in dinoflagellates and provided clues to further explore the molecular underpinnings underlying resting cyst production and broad temperature tolerance of this group of HABs contributors. |
| 资助项目 | Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q09] ; National Science Foundation of China[41606126] ; National Science Foundation of China[61533011] ; National Science Foundation of China[41776125] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000592774900001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/169282]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Tang, Ying Zhong |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Yunyan,Hu, Zhangxi,Shang, Lixia,et al. Transcriptional Responses of the Heat Shock Protein 20 (Hsp20) and 40 (Hsp40) Genes to Temperature Stress and Alteration of Life Cycle Stages in the Harmful Alga Scrippsiella trochoidea (Dinophyceae)[J]. BIOLOGY-BASEL,2020,9(11):17. |
| APA | Deng, Yunyan,Hu, Zhangxi,Shang, Lixia,Chai, Zhaoyang,&Tang, Ying Zhong.(2020).Transcriptional Responses of the Heat Shock Protein 20 (Hsp20) and 40 (Hsp40) Genes to Temperature Stress and Alteration of Life Cycle Stages in the Harmful Alga Scrippsiella trochoidea (Dinophyceae).BIOLOGY-BASEL,9(11),17. |
| MLA | Deng, Yunyan,et al."Transcriptional Responses of the Heat Shock Protein 20 (Hsp20) and 40 (Hsp40) Genes to Temperature Stress and Alteration of Life Cycle Stages in the Harmful Alga Scrippsiella trochoidea (Dinophyceae)".BIOLOGY-BASEL 9.11(2020):17. |
入库方式： OAI收割
来源：海洋研究所
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