Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera
文献类型：期刊论文
| 作者 | Wang, Yu2; Liu, Feng1,2,4; Liu, Manman2; Shi, Shitao2; Bi, Yuping3; Chen, Nansheng1,2,4 |
| 刊名 | GENETICA
 |
| 出版日期 | 2021-01-15 |
| 页码 | 10 |
| 关键词 | Ulva prolifera Carbonic anhydrase Environmental factor Green tide Transcriptional regulation |
| ISSN号 | 0016-6707 |
| DOI | 10.1007/s10709-020-00112-4 |
| 通讯作者 | Liu, Feng(liufeng@qdio.ac.cn) |
| 英文摘要 | Ulva prolifera O.F. Muller (Ulvophyceae, Chlorophyta) is well known as a typical green-tide forming macroalga which has caused the world's largest macroalgal blooms in the Yellow Sea of China. In this study, two full-length gamma-carbonic anhydrase (gamma-CA) genes (Up gamma CA1 and Up gamma CA2) were cloned from U. prolifera. Up gamma CA1 has three conserved histidine residues, which act as an active site for binding a zinc metal ion. In Up gamma CA2, two of the three histidine residues were replaced by serine and arginine, respectively. The two gamma-CA genes are clustered together with other gamma-CAs in Chlorophyta with strong support value (100% bootstrap) in maximum likelihood (ML) phylogenetic tree. Quantitative real-time PCR (qRT-PCR) analysis showed that stressful environmental conditions markedly inhibited transcription levels of these two gamma-CA genes. Low pH value (pH 7.5) significantly increased transcription level of Up gamma CA2 not Up gamma CA1 at 12 h, whereas high pH value (pH 8.5) significantly inhibited the transcription of these two gamma-CA genes at 6 h. These findings enhanced our understanding on transcriptional regulation of gamma-CA genes in response to environmental factors in U. prolifera. |
| 资助项目 | National Natural Science Foundation of China[41876165] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA23050302/XDA23050403/XDB42000000] ; Key Research Program of Frontier Sciences, Chinese Academy of Sciences[QYZDB-SSW-DQC023] ; Major Scientific and Technological Innovation Project of Shandong Province[2019JZZY020706] ; Earmarked Fund for Modern Agro-industry Technology Research System in Shandong Province of China[SDAIT-26-09] |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000607995300001 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/169971]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Liu, Feng |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 3.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol Qingdao, Marine Ecol & Environm Sci Lab, Qingdao 266237, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yu,Liu, Feng,Liu, Manman,et al. Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera[J]. GENETICA,2021:10. |
| APA | Wang, Yu,Liu, Feng,Liu, Manman,Shi, Shitao,Bi, Yuping,&Chen, Nansheng.(2021).Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera.GENETICA,10. |
| MLA | Wang, Yu,et al."Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera".GENETICA (2021):10. |
入库方式： OAI收割
来源：海洋研究所
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