Formalin-Fixed Fish Larvae Could Be Effectively Identified by DNA Barcodes: A Case Study on Thousands of Specimens in South China Sea
文献类型：期刊论文
| 作者 | Hou, Gang3; Chen, Yanying3; Wang, Sijin3; Wang, Jinrun3; Chen, Weitao5; Zhang, Hui1,2,4
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| 刊名 | FRONTIERS IN MARINE SCIENCE
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| 出版日期 | 2021-03-25 |
| 卷号 | 8页码:9 |
| 关键词 | fish larvae DNA barcode South China Sea spawning ground fish conservation |
| DOI | 10.3389/fmars.2021.634575 |
| 通讯作者 | Chen, Weitao(ncuskchenweitao@163.com) ; Zhang, Hui(zhanghui@qdio.ac.cn) |
| 英文摘要 | Delimiting ichthyoplankton is fundamental work for monitoring the recruitment process and identifying the spawning and nursing grounds of fishes. Nevertheless, it is extremely difficult to identify the fish during the early stages at the species level based on morphological characters because of the paucity of diagnostic features. In this study, we investigated the fish larval community through large-scale ecosystemic sampling in South China Sea (SCS) during 2013 and 2017 using DNA barcodes. To maintain the morphologies of fish larvae, we preserved the larvae in formalin and developed a technique to recover their DNA. Among the 3,500 chosen larvae, we successfully extracted DNA from 2,787 larval samples and obtained 1,006 high-quality sequences. Blast searches showed that 408 larvae (i.e., 40.5%) could be unambiguously identified to species, 413 larvae (i.e., 41.1%) were ambiguously species delimitation, and 185 larvae (i.e., 18.4%) showed a low match similarity with target sequences. A total of 101 species were identified, among which 38 and 33 species corresponded to demersal and reef-associated species, whereas the remaining 30 species corresponded to benthopelagic, pelagic-oceanic, bathypelagic, and pelagic-neritic species. High-quality larval photographs of the 101 diagnosed species showed intact morphological characters and thus provided a reference for identifying fish species during the early stages based on morphological characters. Our study highlighted the possibility of recovering and amplifying DNA from formalin-fixed samples and provided new insight into the fish larval community in the SCS. |
| 资助项目 | National Natural Science Foundation of China[31702347] ; National Natural Science Foundation of China[42090044] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Special Fund for Economic Development of the Marine Economy of Guangdong Province[GDME-2018E004] ; undergraduate innovation and Entrepreneurship training program of Guangdong province[S202010566005] ; Guangdong Ocean University[R19006] ; S&T Program of Fangchenggang[Fangke AB20014024] ; Youth Innovation Promotion Association CAS |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000637884700001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/170476]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Chen, Weitao; Zhang, Hui |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 3.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 5.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Gang,Chen, Yanying,Wang, Sijin,et al. Formalin-Fixed Fish Larvae Could Be Effectively Identified by DNA Barcodes: A Case Study on Thousands of Specimens in South China Sea[J]. FRONTIERS IN MARINE SCIENCE,2021,8:9. |
| APA | Hou, Gang,Chen, Yanying,Wang, Sijin,Wang, Jinrun,Chen, Weitao,&Zhang, Hui.(2021).Formalin-Fixed Fish Larvae Could Be Effectively Identified by DNA Barcodes: A Case Study on Thousands of Specimens in South China Sea.FRONTIERS IN MARINE SCIENCE,8,9. |
| MLA | Hou, Gang,et al."Formalin-Fixed Fish Larvae Could Be Effectively Identified by DNA Barcodes: A Case Study on Thousands of Specimens in South China Sea".FRONTIERS IN MARINE SCIENCE 8(2021):9. |
入库方式： OAI收割
来源：海洋研究所
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