A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria
文献类型：期刊论文
| 作者 | Li, Mengna1,3,4,5; Chen, Hao2,3,4,5,6 ; Wang, Minxiao2,3,4,5,6; Zhong, Zhaoshan2,3,4,5,6; Wang, Hao2,3,4,5,6; Zhou, Li2,3,4,5,6; Zhang, Huan2,3,4,5,6; Li, Chaolun1,2,3,4,5,6
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| 刊名 | PEERJ
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| 出版日期 | 2021-04-30 |
| 卷号 | 9页码:21 |
| 关键词 | Gigantidas platifrons Methane-oxidizing bacteria Symbiosis Immune recognition Toll like receptor Pattern recognition receptors |
| ISSN号 | 2167-8359 |
| DOI | 10.7717/peerj.11282 |
| 通讯作者 | Li, Chaolun(lcl@qdio.ac.cn) |
| 英文摘要 | Symbiosis with chemosynthetic bacteria is an important ecological strategy for the deep-sea megafaunas including mollusks, tubeworms and crustacean to obtain nutrients in hydrothermal vents and cold seeps. How the megafaunas recognize symbionts and establish the symbiosis has attracted much attention. Bathymodiolinae mussels are endemic species in both hydrothermal vents and cold seeps while the immune recognition mechanism underlying the symbiosis is not well understood due to the nonculturable symbionts. In previous study, a lipopolysaccharide (LPS) pull-down assay was conducted in Gigantidas platifrons to screen the pattern recognition receptors potentially involved in the recognition of symbiotic methane-oxidizing bacteria (MOB). Consequently, a total of 208 proteins including GpTLR13 were identified. Here the molecular structure, expression pattern and immune function of GpTLR13 were further analyzed. It was found that GpTLR13 could bind intensively with the lipid A structure of LPS through surface plasmon resonance analysis. The expression alternations of GpTLR13 transcripts during a 28-day of symbiont-depletion assay were investigated by real-time qPCR. As a result, a robust decrease of GpTLR13 transcripts was observed accompanying with the loss of symbionts, implying its participation in symbiosis. In addition, GpTLR13 transcripts were found expressed exclusively in the bacteriocytes of gills of G. platifrons by in situ hybridization. It was therefore speculated that GpTLR13 may be involved in the immune recognition of symbiotic methane-oxidizing bacteria by specifically recognizing the lipid A structure of LPS. However, the interaction between GpTLR13 and symbiotic MOB was failed to be addressed due to the nonculturable symbionts. Nevertheless, the present result has provided with a promising candidate as well as a new approach for the identification of symbiont-related genes in Bathymodiolinae mussels. |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050303] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42020401] ; Key Research Program of Frontier Sciences, CAS[ZDBS-LY-DQC032] ; National Key Research and Development Program of China[2018YFC0310800] ; National Natural Science Foundation of China[42030407] ; National Natural Science Foundation of China[42076091] ; National Natural Science Foundation of China[41906103] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, CAS[COMS2020Q02] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000647021700004 |
| 出版者 | PEERJ INC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/170916]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 海洋研究所_深海极端环境与生命过程研究中心 |
| 通讯作者 | Li, Chaolun |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci CODR, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, KLMEES, Qingdao, Peoples R China 5.Chinese Acad Sci, Ctr Deep Sea Res, Qingdao, Peoples R China 6.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Mengna,Chen, Hao,Wang, Minxiao,et al. A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria[J]. PEERJ,2021,9:21. |
| APA | Li, Mengna.,Chen, Hao.,Wang, Minxiao.,Zhong, Zhaoshan.,Wang, Hao.,...&Li, Chaolun.(2021).A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria.PEERJ,9,21. |
| MLA | Li, Mengna,et al."A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria".PEERJ 9(2021):21. |
入库方式： OAI收割
来源：海洋研究所
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