Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization
文献类型：期刊论文
| 作者 | Zhan, Zifeng1,2,4; Xu, Kuidong1,2,3,4
|
| 刊名 | ACTA OCEANOLOGICA SINICA
 |
| 出版日期 | 2020-12-28 |
| 页码 | 5 |
| 关键词 | oligonucleotide probes molecular methods scuticociliates SSU rRNA abalone mollusc |
| ISSN号 | 0253-505X |
| DOI | 10.1007/s13131-020-1683-0 |
| 通讯作者 | Xu, Kuidong(kxu@qdio.ac.cn) |
| 英文摘要 | The scuticociliatid ciliates Ancistrum haliotis and A. crassum are parasites that may cause high mortality in the cultured abalone Haliotis spp. and the bivalve Ruditapes philippinarum. Traditional identification with silver staining methods is hampered by their morphological similarities to closely related species and the complicated procedures of morphological analysis. We designed two SSU rRNA-targeted oligonucleotide probes labeled with a fluorochrome, and optimized the fluorescence in situ hybridization (FISH) protocols for identification of A. halioti and A. crassum, respectively. The assays resulted in a clear identification by strong fluorescence signals from the oligonucleotide probes. The method can be used for quick and accurate quantitative analysis of A. haliotis and A. crassum infections on host molluscs. |
| 资助项目 | National Natural Science Foundation of China[41876171] ; National Natural Science Foundation of China[41406171] |
| WOS研究方向 | Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:000603188400002 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/169548]  |
| 专题 | 海洋研究所_海洋生物分类与系统演化实验室 |
| 通讯作者 | Xu, Kuidong |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhan, Zifeng,Xu, Kuidong. Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization[J]. ACTA OCEANOLOGICA SINICA,2020:5. |
| APA | Zhan, Zifeng,&Xu, Kuidong.(2020).Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization.ACTA OCEANOLOGICA SINICA,5. |
| MLA | Zhan, Zifeng,et al."Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization".ACTA OCEANOLOGICA SINICA (2020):5. |
入库方式： OAI收割
来源：海洋研究所
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