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DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation

文献类型：期刊论文

作者Zeng, Yan1,3,4; Qi, Peng1,3,4; Wang, Yingwen1,2,3,4; Chen, Chao1,3,4; Zhang, Dun1,3,4
刊名BIOSENSORS & BIOELECTRONICS
出版日期2021-04-01
卷号177页码:9
关键词DNA nanostructure Pathogenic bacteria Ultrasensitive detection Bacteria inactivation Antibiotics loading
ISSN号0956-5663
DOI10.1016/j.bios.2021.112982
通讯作者Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
英文摘要Pathogenic bacteria levels are significantly related with disease control, clinical diagnosis, and even environmental monitoring. It is becoming highly urgent to achieve ultrasensitive detection of pathogenic bacteria and efficient combat of bacterial infection. Toward this end, we have assembled a DNA Pom-Pom nanostructure (PP-N) based multifunctional platform for pathogenic bacteria determination and inactivation. In particular, one DNA oligonucleotide probe that serve as a trigger was specifically designed for the autonomous cross-opening of metastable DNA hairpin probes and long dsDNA structure formation, achieving a catalytic self-assembly of DNA nanostructure. Numerous DNA strands in this PP-N assembly provide sufficient interaction sites for functional domains and connector, showing high programmability, excellent biostability, as well as selective target recognition. With these properties, the fluorescence dyes modified PP-N platform showed excellent bacteria analysis with both excellent selectivity and ultrasensitive determination limit as low as 2.0 CFU/mL. Furthermore, the aptamer-functionalized and antibiotics loaded PP-N platform demonstrate excellent merits of high antibiotics-loading capacity and negligible cytotoxicity to targets. Therefore, this DNA PP-N assembly based multifunctional platform promise its great application in targeted sensing, combating bacterial infection, and potential clinic therapy.
资助项目National Natural Science Foundation of China[41906037] ; National Natural Science Foundation of China[41876101] ; Young Elite Scientists Sponsorship Program by CAST[20180276]
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种英语
WOS记录号WOS:000618826100001
出版者ELSEVIER ADVANCED TECHNOLOGY
源URL[http://ir.qdio.ac.cn/handle/337002/169606]  
专题海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者Qi, Peng; Zhang, Dun
作者单位1.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
推荐引用方式
GB/T 7714		 Zeng, Yan,Qi, Peng,Wang, Yingwen,et al. DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation[J]. BIOSENSORS & BIOELECTRONICS,2021,177:9.
APA Zeng, Yan,Qi, Peng,Wang, Yingwen,Chen, Chao,&Zhang, Dun.(2021).DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation.BIOSENSORS & BIOELECTRONICS,177,9.
MLA Zeng, Yan,et al."DNA porn-porn nanostructure as a multifunctional platform for pathogenic bacteria determination and inactivation".BIOSENSORS & BIOELECTRONICS 177(2021):9.

入库方式： OAI收割

来源：海洋研究所

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