中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform

文献类型:期刊论文

作者Song, Fengge3; Wei, Yangdao3; Wang, Peng1; Ge, Xiaolin3; Li, Chaoyang3; Wang, Aimin3; Yang, Zhiqing3; Wan, Yi1,2,3; Li, Jinghong4
刊名BIOSENSORS & BIOELECTRONICS
出版日期2021-08-01
卷号185页码:5
ISSN号0956-5663
关键词Bacterial diagnostics CRISPR-Cas14a Tag-specific primer extension Magneto-DNA
DOI10.1016/j.bios.2021.113262
通讯作者Yang, Zhiqing(yangzhiqing@hainanu.edu.cn) ; Wan, Yi(993602@hainu.edu.cn)
英文摘要Nucleic acid-based diagnosis using CRISPR-Cas associated enzymes is essential for rapid infectious disease diagnosis and treatment strategies during a global pandemic. The obstacle has been blossomed CRIPSR-Cas based tools that can monitor wide range of pathogens in clinical samples with ultralow concentrations. Here, a universal nucleic acid magneto-DNA nanoparticle system was exploited for the detection of pathogenic bacteria, based on the collateral cleavage activity of CRISPR-Cas14a and tag-specific primer extension. In the system, the target nucleic acids were amplificated and be separated from mixtures by streptavidin-coated magnetic bead. The collateral cleavage activity of CRISPR-Cas14a can be activated via the tag sequence on the target product. Consequently, the fluorophore quencher reporter can be activated by CRISPR-Cas14a, leading to the increasing response. The exploited universal bacterial diagnostic can distinguish six different bacteria strains with 1 cfu/mL or 1 aM sensitivity, which may provide new strategies to construct fast, accurate, cost-effective and sensitive diagnostic tools in environments with limited resources.
资助项目Hainan Provincial Natural Science Foundation of China[420RC518] ; Open Fund of Shandong Key Laboratory of Corrosion Science[KLCS201910] ; Tsinghua University Initiative Scientific Research Program[2020Z99CFZ019] ; Tsinghua University Spring Breeze Fund[2020Z99CFZ019] ; Research Foundation of Hainan University[KYQD (ZR) 1711] ; Research Foundation of Hainan University[KYQD (ZR) 1997] ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology[ZD202003KF04] ; Open fund of Fujian Provincial Key Laboratory of Photonics Technology[JYG 2002]
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种英语
出版者ELSEVIER ADVANCED TECHNOLOGY
WOS记录号WOS:000655570200001
源URL[http://ir.qdio.ac.cn/handle/337002/171001]  
专题海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者Yang, Zhiqing; Wan, Yi
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Bio Fouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
3.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China
4.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China
推荐引用方式
GB/T 7714
Song, Fengge,Wei, Yangdao,Wang, Peng,et al. Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform[J]. BIOSENSORS & BIOELECTRONICS,2021,185:5.
APA Song, Fengge.,Wei, Yangdao.,Wang, Peng.,Ge, Xiaolin.,Li, Chaoyang.,...&Li, Jinghong.(2021).Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform.BIOSENSORS & BIOELECTRONICS,185,5.
MLA Song, Fengge,et al."Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform".BIOSENSORS & BIOELECTRONICS 185(2021):5.

入库方式: OAI收割

来源:海洋研究所

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