A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture
文献类型：期刊论文
| 作者 | Zhao, Yan1,3,4,5 ; Jiang, Shuai1,3,4; Zhang, Jian1,2,3,4; Guan, Xiao-Lu1,3,4; Sun, Bo-Guang1,3,4; Sun, Li1,3,4
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| 刊名 | VIRULENCE
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| 出版日期 | 2021 |
| 卷号 | 12期号:1页码:1362-1376 |
| 关键词 | Bacillus cereus deep-sea cold seep infection NLRP3 inflammasome pyroptosis |
| ISSN号 | 2150-5594 |
| DOI | 10.1080/21505594.2021.1926649 |
| 通讯作者 | Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | Recent studies indicate that the Bacillus species is distributed in deep-sea environments. However, no specific studies on deep-sea Bacillus cereus have been documented. In the present work, we isolated a B. cereus strain, H2, from the deep-sea cold seep in South China Sea. We characterized the pathogenic potential of H2 and investigated H2-induced death of different types of cells. We found that H2 was capable of tissue dissemination and causing acute mortality in mice and fish following intraperitoneal/intramuscular injection. In vitro studies revealed that H2 infection of macrophages induced pyroptosis and activation of the NLRP3 inflammasome pathway that contributed partly to cell death. H2 infection activated p38, JNK, and ERK, but only JNK proved to participate in H2-triggered cell death. Reactive oxygen species (ROS) and intracellular Ca2+ were essential to H2-induced activation of JNK and NLRP3 inflammasome. In contrast, lysosomal rupture and cathepsins were required for H2-induced NLRP3 inflammasome activation but not for JNK activation. This study revealed for the first time the virulence characteristics of deep-sea B. cereus and provided new insights into the mechanism of B. cereus infection. |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403] ; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences[KLMVI-OP-202002] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Immunology ; Infectious Diseases ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000652027500001 |
| 出版者 | TAYLOR & FRANCIS INC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/170955]  |
| 专题 | 海洋研究所_深海极端环境与生命过程研究中心 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, Deep Sea Res Ctr, Inst Oceanol, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yan,Jiang, Shuai,Zhang, Jian,et al. A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture[J]. VIRULENCE,2021,12(1):1362-1376. |
| APA | Zhao, Yan,Jiang, Shuai,Zhang, Jian,Guan, Xiao-Lu,Sun, Bo-Guang,&Sun, Li.(2021).A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture.VIRULENCE,12(1),1362-1376. |
| MLA | Zhao, Yan,et al."A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture".VIRULENCE 12.1(2021):1362-1376. |
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来源：海洋研究所
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