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Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography

文献类型：期刊论文

作者Luo, Chunhua1,4; Lu, Dongyuan1,2,4; Zheng, Lu1,2,4; Zhang, Fan1,2,4; Zhang, Xiao1,2,4; Lu, Shouqin1,2,4; Zhang, Chen1,4; Jia, Xiaohua3; Shu, Xinyu1,2,4; Li, Peiwen1,4
刊名BIOMATERIALS SCIENCE
出版日期2021-04-20
页码15
ISSN号2047-4830
DOI10.1039/d1bm00174d
通讯作者Long, Mian(mlong@imech.ac.cn)
英文摘要Mechanical or physical cues are associated with the growth and differentiation of embryonic stem cells (ESCs). While the substrate stiffness or topography independently affects the differentiation of ESCs, their cooperative regulation on lineage-specific differentiation remains largely unknown. Here, four topographical configurations on stiff or soft polyacrylamide hydrogel were combined to direct hepatic differentiation of human H1 cells via a four-stage protocol, and the coupled impacts of stiffness and topography were quantified at distinct stages. Data indicated that the substrate stiffness is dominant in stemness maintenance on stiff gel and hepatic differentiation on soft gel while substrate topography assists the differentiation of hepatocyte-like cells in positive correlation with the circularity of H1 clones initially formed on the substrate. The differentiated cells exhibited liver-specific functions such as maintaining the capacities of CYP450 metabolism, glycogen synthesis, ICG engulfment, and repairing liver injury in CCl4-treated mice. These results implied that the coupling of substrate stiffness and topography, combined with the biochemical signals, is favorable to improve the efficiency and functionality of hepatic differentiation of human ESCs.
资助项目National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[31870931] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31470907] ; National Natural Science Foundation of China[81703012] ; Chinese Science Academy[QYZDJ-SSW-JSC018] ; Chinese Science Academy[GJJSTU20190005] ; Chinese Science Academy[XDB22040101]
WOS研究方向Materials Science
语种英语
WOS记录号WOS:000641302300001
出版者ROYAL SOC CHEMISTRY
资助机构National Natural Science Foundation of China ; Chinese Science Academy
源URL[http://ir.ia.ac.cn/handle/173211/44266]  
专题自动化研究所_中国科学院分子影像重点实验室
通讯作者Long, Mian
作者单位1.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
4.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Luo, Chunhua,Lu, Dongyuan,Zheng, Lu,et al. Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography[J]. BIOMATERIALS SCIENCE,2021:15.
APA Luo, Chunhua.,Lu, Dongyuan.,Zheng, Lu.,Zhang, Fan.,Zhang, Xiao.,...&Long, Mian.(2021).Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography.BIOMATERIALS SCIENCE,15.
MLA Luo, Chunhua,et al."Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography".BIOMATERIALS SCIENCE (2021):15.

入库方式： OAI收割

来源：自动化研究所

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