Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N
文献类型：期刊论文
| 作者 | Yao, BL; Zheng, YA; Wang, YL; Lei, M; Chen, GF; Hampp, N |
| 刊名 | optics communications
 |
| 出版日期 | 2003-03-15 |
| 卷号 | 218期号:1-3页码:125-130 |
| 关键词 | bacteriorhodopsin BR-D96N photochromic kinetic spectra |
| ISSN号 | 0030-4018 |
| 通讯作者 | yao baoli |
| 英文摘要 | br-d96n is a kind of genetically site-specific mutants of bacteriorhodopsin (br) with obvious photochromic effect. compared to the wild type br, the lifetime of m state of br-d96n is prolonged to several minutes so that the photochromic kinetics and the intermediates formation can be studied by the conventional spectra analysis. in the experiment, the absorption spectra of the sample at different time after light illumination are measured with spectrophotometer. by fitting and analyzing the variation of the spectra, we suppose that there are three main states in the, photochromic process, i.e., b state (light-adapted state), m state and d state (dark-adapted state). the absorption spectra of the b state, m state and d state are extracted from the experimental data based on this three-state model and the spectra at various time are fitted with the least-square method. so, the variations of population percentages of the m state, b state and d state are obtained and the m state and b state lifetimes are estimated. in another way, from the measurement of the absorption dynamics at 407 and 568 nm, the m state and b state lifetimes are also obtained by two exponential data fitting, which give coincident results with those of the spectra analysis. (c) 2003 elsevier science b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 光学 |
| 类目[WOS] | optics |
| 研究领域[WOS] | optics |
| 关键词[WOS] | bacteriorhodopsin |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000181641000017 |
| 公开日期 | 2010-01-12 |
| 源URL | [http://ir.opt.ac.cn/handle/181661/6478]  |
| 专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt Technol, Xian 710068, Peoples R China 2.Univ Marburg, Inst Chem Phys, D-35032 Marburg, Germany |
| 推荐引用方式 GB/T 7714 | Yao, BL,Zheng, YA,Wang, YL,et al. Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N[J]. optics communications,2003,218(1-3):125-130. |
| APA | Yao, BL,Zheng, YA,Wang, YL,Lei, M,Chen, GF,&Hampp, N.(2003).Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N.optics communications,218(1-3),125-130. |
| MLA | Yao, BL,et al."Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N".optics communications 218.1-3(2003):125-130. |
入库方式： OAI收割
来源：西安光学精密机械研究所
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