Molecular characterization and subcellular localization of Carassius auratus interferon regulatory factor-1
文献类型：期刊论文
| 作者 | Shi, Yan; Zhang, Yi-Bing; Zhao, Zhe; Jiang, Jun; Zhang, Qi-Ya; Gui, Jian-Fang |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2008 |
| 卷号 | 32期号:2页码:134-146 |
| 关键词 | interferon regulator factor-1 (IRF-1) Carassius auratus grass carp hemorrhagic virus inductive expression nuclear localization signal (NLS) subcellular localization |
| ISSN号 | 0145-305X |
| DOI | 10.1016/i.dci.2007.05.004 |
| 通讯作者 | Gui, Jian-Fang(jfgui@ihb.ac.cn) |
| 英文摘要 | Interferon (IFN)-regulatory transcription factor-1 (IRF-1) has been studied in mammals and fish but little is known about the relationship between its gene structure and nuclear 'ion of IRF-1 protein. In this study, a cDNA encoding Carassius auratus IRF-1 (CaIRF-1) was isolated from an interferon-producing cell line, C. ouratus blastulae embryonic (CAB) cells, exposed to UV-inactivated grass carp hemorrhagic virus (GCHV). The CaIRF-1 genomic locus exhibits exon-intron arrangements similar to those of other vertebrate IRF-1 loci, with nine exons and eight introns, although together with pufferfish IRF-1, CaIRF-1 distinguishes itself from other vertebrate IRF-1 genes by a relatively compact genomic size. Similar to the known IRF-1 genes, CaIRF-1 is ubiquitously expressed, and is upregulated in vitro and in vivo in response to virus, Poty I:C, or CAB INF-containing supernatant (ICS). Subcellular localization analysis confirms the nuclear distribution of CaIRF-1 protein, and reveals two nuclear localization signals (NILS), any one of which is sufficient for nuclear translocation of CaIRF-1. One NLS Locates to amino acids 117-146, and appears to be the structural and functional equivalent of the NLS in mammalian IRF-1. The second NLS (amino acids 73-115) is found within the DNA-binding domain (DBD) of CaIRF-1, and contains two regions rich in basic amino acids (''(KDKSINK101)-K-95" and ''(75)KTWKANFR(82)"). In comparison with mammalian IRF-1, in which the corresponding amino acid stretch does not seem to drive nuclear translocation, five conserved basic amino acids (K-75, K-78, R-82, K-95, and K-101) and one non-conserved basic amino acid (K-97) are present in this NLS from CaIRF-1. This observation suggests that K97 Of CaIRF-1 might be essential for the function of its second NLS, wherein the six basic aminoacids might cooperate to drive CaIRF-1 to the nucleus. Therefore, the current study has revealed a new nuclear localization motif in the DBD of a vertebrate IRF-1. (C) 2007 Elsevier Ltd. All rights reserved. |
| WOS研究方向 | Immunology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000252062400006 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.imr.ac.cn/handle/321006/92818]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Gui, Jian-Fang |
| 作者单位 | Chinese Acad Sci, Grad Sch Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yan,Zhang, Yi-Bing,Zhao, Zhe,et al. Molecular characterization and subcellular localization of Carassius auratus interferon regulatory factor-1[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2008,32(2):134-146. |
| APA | Shi, Yan,Zhang, Yi-Bing,Zhao, Zhe,Jiang, Jun,Zhang, Qi-Ya,&Gui, Jian-Fang.(2008).Molecular characterization and subcellular localization of Carassius auratus interferon regulatory factor-1.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,32(2),134-146. |
| MLA | Shi, Yan,et al."Molecular characterization and subcellular localization of Carassius auratus interferon regulatory factor-1".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 32.2(2008):134-146. |
入库方式： OAI收割
来源：金属研究所
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