Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo
文献类型：期刊论文
| 作者 | Zhang, Ying1,2; Wang, Wei1; Xie, Yubing1; Yu, Weiting1; Lv, Guojun1,2; Guo, Xin1; Xiong, Ying1; Ma, Xiaojun1 |
| 刊名 | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
 |
| 出版日期 | 2008 |
| 卷号 | 84B期号:1页码:79-88 |
| 关键词 | microencapsulation recombinant cell cell growth endostatin production stability in vivo |
| ISSN号 | 1552-4973 |
| 通讯作者 | Ma, Xiaojun(maxj@dicp.ae.en) |
| 英文摘要 | Microencapsulation of recombinant cells secreting endostatin offers a promising approach to tumor gene therapy in which therapeutic protein is delivered in a sustainable and long-term fashion by encapsulated recombinant cells. However, the studies of cell growth and protein production in vivo are very limited. In this study, the effects of microencapsulation parameters on in vivo cell growth, endostatin production, and microcapsule stability after implantation in the peritoneal cavity of mice were for the first time investigated. Microcapsules with liquid core reached higher cell density and endostatin production at day 18 than microcapsules with solid core. There was no significant difference in stability whether the core of the microcapsulle was solid or liquid. Decrease in microcapsule size increased the stability of microcapsule. The microcapsules kept intact in the peritoneal cavity of mice after 36 days of implantation when the microcapsules size was 240 mu m in diameter, which gave rise to high endostatin production as well. The optimized microencapsulation conditions for in vivo implantation are liquid core and 240 mu m in diameter. This study provides useful information for antiangiogenic gene therapy to tumors using microencapsulated recombinant cells. (c) 2007 Wiley Periodicals, Inc. |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000251802900010 |
| 出版者 | WILEY-LISS |
| 源URL | [http://ir.imr.ac.cn/handle/321006/93233]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Ma, Xiaojun |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Ying,Wang, Wei,Xie, Yubing,et al. Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,2008,84B(1):79-88. |
| APA | Zhang, Ying.,Wang, Wei.,Xie, Yubing.,Yu, Weiting.,Lv, Guojun.,...&Ma, Xiaojun.(2008).Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,84B(1),79-88. |
| MLA | Zhang, Ying,et al."Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 84B.1(2008):79-88. |
入库方式： OAI收割
来源：金属研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。