Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains
文献类型：期刊论文
| 作者 | Zhu, Lili1,2; Yuan, Cai1,2; Chen, Zhuo1,2; Wang, Wanyu2,3; Huang, Mingdong1,2 |
| 刊名 | TOXICON
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 55期号:2-3页码:375-380 |
| 关键词 | Jerdonitin Snake venom metalloproteinases Recombinant protein expression Metalloproteinase Disintegrin Fibrinolysis activity Platelet aggregation Tumor cells MTT colorimetric assay Pichia pastoris |
| ISSN号 | 0041-0101 |
| DOI | 10.1016/j.toxicon.2009.08.016 |
| 通讯作者 | Huang, Mingdong(mhuang@fjirsm.ac.cn) |
| 英文摘要 | Jerdonitin is a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains. In this study, we established a high-level expression system in Pichia pastoris and developed a purification strategy for the recombinant Jerdonitin. This recombinant Jerdonitin degraded fibrinogen at a level of activity comparable with its wild type. The effects of recombinant Jerdonitin on inhibiting ADP-induced human platelet aggregation were in a dose-dependent manner with an IC(50) of 248 nM. In addition, we reported here that Jerdonitin can significantly inhibit the growth of several cell lines, including human liver cancer cells (Bel7402), human leukemia cells (K562) and human gastric carcinoma cells (BGC823). This study offers recombinant Jerdonitin that will be valuable for further functional and structural studies of Jerdonitin. (C) 2009 Elsevier Ltd. All rights reserved. |
| 资助项目 | CAS[KSCX2YW-R-082] ; Fujian NSF[200910029] ; NSFC[30625011] ; NSFC[30811130467] ; NSFC[30770429] ; MOST[2007CB914304] ; MOST[2006AA02A313] |
| WOS研究方向 | Pharmacology & Pharmacy ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000274579100026 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 资助机构 | CAS ; Fujian NSF ; NSFC ; MOST |
| 源URL | [http://ir.imr.ac.cn/handle/321006/101134]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Huang, Mingdong |
| 作者单位 | 1.Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fuzhou 350002, Peoples R China 2.Chinese Acad Sci, Grad Sch Chinese Acad Sci, Beijing 10039, Peoples R China 3.Chinese Acad Sci, Kunming Inst Zool, Dept Anim Toxinol, Kunming 650223, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Lili,Yuan, Cai,Chen, Zhuo,et al. Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains[J]. TOXICON,2010,55(2-3):375-380. |
| APA | Zhu, Lili,Yuan, Cai,Chen, Zhuo,Wang, Wanyu,&Huang, Mingdong.(2010).Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains.TOXICON,55(2-3),375-380. |
| MLA | Zhu, Lili,et al."Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains".TOXICON 55.2-3(2010):375-380. |
入库方式： OAI收割
来源：金属研究所
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