Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms
文献类型：期刊论文
| 作者 | Zhu, BG; Chen, YZ; Xing, BR |
| 刊名 | JOURNAL OF NEURAL TRANSMISSION
 |
| 出版日期 | 1999 |
| 卷号 | 106期号:3-4页码:257-264 |
| 关键词 | glutamate uptake Ca2+ synaptosome Na+ channel calmodulin Na2+/calmodulin-dependent protein kinase II |
| ISSN号 | 0300-9564 |
| 通讯作者 | Zhu, BG() |
| 英文摘要 | The effects of Ca2+ on glutamate uptake by synaptosomes were investigated. Glutamate uptake was decreased in the absence of Ca2+ with 2 mM EGTA added to the medium. After a 8-min preincubation, the uptake reduced to the greatest extent (about 64% of the uptake measured in Ca2+ medium). According to the kinetic analysis, lack of Ca2+ resulted in a reduction of the Vmax for glutamate uptake. These results suggested external Ca2+ was necessary for the optimal uptake of glutamate. TTX, an inhibitor of the voltage-dependent Na+ channel, almost reversed the reduction of glutamate uptake, indicating that the uptake might be impaired via the partial activation of the voltage-dependent tetrodotoxin-sensitive Na+ channel. On the other hand, glutamate uptake was decreased by trifluoperazine, a calmodulin antagonist, and KN-62, an inhibtor of Ca2+/calmodulin-dependent protein kinase II, implying that intrasynaptosomal Ca2+ might also have a role in the uptake of glutamte, and Ca2+/calmodulin-dependent processes, such as Ca2+/calmodulin-dependent protein kinase II, might be involved in the modulation of glutamate transporter activity. |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000079984000004 |
| 出版者 | SPRINGER-VERLAG WIEN |
| 源URL | [http://ir.imr.ac.cn/handle/321006/109867]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Zhu, BG |
| 作者单位 | 1.Second Mil Med Univ, Inst Neurosci, Dept Physiol, Shanghai 200433, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Physiol, Neurobiol Lab, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, BG,Chen, YZ,Xing, BR. Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms[J]. JOURNAL OF NEURAL TRANSMISSION,1999,106(3-4):257-264. |
| APA | Zhu, BG,Chen, YZ,&Xing, BR.(1999).Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms.JOURNAL OF NEURAL TRANSMISSION,106(3-4),257-264. |
| MLA | Zhu, BG,et al."Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms".JOURNAL OF NEURAL TRANSMISSION 106.3-4(1999):257-264. |
入库方式： OAI收割
来源：金属研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。