Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme
文献类型：期刊论文
| 作者 | Hudek, Lee1; Rai, L. C.3; Freestone, David1; Michalczyk, Agnes1; Gibson, Maria1; Song, Y. F.2; Ackland, M. Leigh1 |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 75期号:3页码:784-791 |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/AEM.02481-08 |
| 通讯作者 | Ackland, M. Leigh(leigha@deakin.edu.au) |
| 英文摘要 | Zinc homeostasis was investigated in Nostoc punctiforme. Cell tolerance to Zn(2+) over 14 days showed that ZnCl(2) levels above 22 mu M significantly reduced cell viability. After 3 days in 22 mu M ZnCl(2), ca. 12% of the Zn(2+) was in an EDTA-resistant component, suggesting an intracellular localization. Zinquin fluorescence was detected within cells exposed to concentrations up to 37 mu M relative to 0 mu M treatment. Radiolabeled (65)Zn showed Zn(2+) uptake increased over a 3-day period, while efflux occurred more rapidly within a 3-h time period. Four putative genes involved in Zn(2+) uptake and efflux in N. punctiforme were identified: (i) the predicted Co/Zn/Cd cation transporter, putative CDF; (ii) the predicted divalent heavy-metal cation transporter, putative Zip; (iii) the ATPase component and Fe/Zn uptake regulation protein, putative Fur; and (iv) an ABC-type Mn/Zn transport system, putative zinc ZnuC, ZnuABC system component. Quantitative real-time PCR indicated the responsiveness of all four genes to 22 mu M ZnCl(2) within 3 h, followed by a reduction to below basal levels after 24 h by putative ZIP, ZnuC, and Fur and a reduction to below basal level after 72 h by putative CDF efflux gene. These results demonstrate differential regulation of zinc transporters over time, indicating a role for them in zinc homeostasis in N. punctiforme. |
| 资助项目 | International Science Linkage Programme ; Australia-China Special Fund for Scientific and Technological Cooperation. |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000262690100029 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 资助机构 | International Science Linkage Programme ; Australia-China Special Fund for Scientific and Technological Cooperation. |
| 源URL | [http://ir.imr.ac.cn/handle/321006/126313]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Ackland, M. Leigh |
| 作者单位 | 1.Deakin Univ, Ctr Cellular & Mol Biol, Sch Life & Environm Sci, Burwood, Vic 3125, Australia 2.Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang, Peoples R China 3.Banaras Hindu Univ, CAS Bot, Algal Biol Lab, Mol Biol Unit, Varanasi 221005, Uttar Pradesh, India |
| 推荐引用方式 GB/T 7714 | Hudek, Lee,Rai, L. C.,Freestone, David,et al. Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2009,75(3):784-791. |
| APA | Hudek, Lee.,Rai, L. C..,Freestone, David.,Michalczyk, Agnes.,Gibson, Maria.,...&Ackland, M. Leigh.(2009).Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,75(3),784-791. |
| MLA | Hudek, Lee,et al."Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75.3(2009):784-791. |
入库方式： OAI收割
来源：金属研究所
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