Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation
文献类型：期刊论文
| 作者 | Zhao, Jing; Ren, Ling; Liu, Meixia; Xi, Tong; Zhang, Bingchun; Yang, Ke |
| 刊名 | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 106期号:5页码:2019-2028 |
| 关键词 | Cu bearing stainless steel fibrosis CuZnSOD TGF-beta 1 Smad3 COL1 COL3 |
| ISSN号 | 1552-4973 |
| DOI | 10.1002/jbm.b.34005 |
| 通讯作者 | Ren, Ling(lren@imr.ac.cn) ; Yang, Ke(kyang@imr.ac.cn) |
| 英文摘要 | Recurrent stenosis is the main reason inducing the failure of urethral stricture treatment. Our previous study has found that the 316L type Cu bearing stainless steel (316L-Cu SS) showed antimicrobial activity and anti-encrustation performance when it was used for relieving urethral obstructer. However, whether it can reduce the occurrence of fibrosis or not, we need further investigation to compare the cellular and molecular responses of human urethral scar fibroblast cells (USFCs) on 316L-Cu SS and medical grade 316L stainless (316L SS, as a control). [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulfophenyl)- 2H-tetrazolium (MTS) and Transwell were used to assess the cellular responses, which confirmed that 316L-Cu SS could inhibit proliferation and migration of USFCs. Molecular expressions of fibrosis were evaluated by western blot, real-time quantitative polymerase chain reaction (qPCR), and Cu/Zn superoxide dismutase (CuZnSOD) measurement. The results indicated that up-regulating of CuZnSOD attenuated the transforming growth factor-1 expression and phosphorylation of Smad3 after exposure to 316L-Cu SS. Besides, the content of collagen type I (COL1) and collagen type III (COL3) secreting into the culture medium measured by enzyme-linked immunosorbent assay were in accord with the results of messenger ribonucleic acids. Both of them exhibited lower levels of COL1/COL3 exposure to 316L-Cu SS, demonstrating the inhibitory performance of 316L-Cu SS against fibrosis. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2019-2028, 2018. |
| 资助项目 | National Basic Research Program of China[2012CB619101] ; National Natural Science Foundation of China[51171186] ; Foundation of Jiangsu Collaborative Innovation Center of Biomedical Functional Materials |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000435443500038 |
| 出版者 | WILEY |
| 资助机构 | National Basic Research Program of China ; National Natural Science Foundation of China ; Foundation of Jiangsu Collaborative Innovation Center of Biomedical Functional Materials |
| 源URL | [http://ir.imr.ac.cn/handle/321006/128636]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Ren, Ling; Yang, Ke |
| 作者单位 | Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Jing,Ren, Ling,Liu, Meixia,et al. Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,2018,106(5):2019-2028. |
| APA | Zhao, Jing,Ren, Ling,Liu, Meixia,Xi, Tong,Zhang, Bingchun,&Yang, Ke.(2018).Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,106(5),2019-2028. |
| MLA | Zhao, Jing,et al."Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 106.5(2018):2019-2028. |
入库方式： OAI收割
来源：金属研究所
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